An activity directed fractionation of a 50% aqueous ethanol extract of A. wilkesiana and A. hispida leaves resulted in the isolation of gallic acid, corilagin and geraniin as the compounds responsible for the observed antimicrobial activity. Quercetin 3-O-rutinoside and kaempferol 3-O-rutinoside were also isolated from the inactive fraction of A. hispida. The structures were established by permethylation, 2D - NMR ((1)H and (13)C) and MS data.
antimicrobial, Acalypha wilkesiana, Acalypha hispida, corilagin, geraniin
NCBI PubMed ID: 10925407Publication DOI: 10.1002/1099-1573(200008)14:5<371::aid-ptr625>3.0.co;2-fJournal NLM ID: 8904486Publisher: Chichester: Wiley
Institutions: Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria, Institute de Chimie des Substances Naturelles, Centre National de la Recherche Scientific, Gif sur Yvette, France
Methods: 13C NMR, 1H NMR, methylation, TLC, ESI-MS, biological assays, extraction, optical rotation measurement, CC, evaporation, antimicrobial assay