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The hypoglycemic effect of water as well as butanolic extracts prepared from aerial parts of Equisetum myriochaetum (Equisetaceae) was examined in streptozotocin induced diabetic rats. A single oral administration of the water extract (WE) at doses of 7 and 13 mg/kg and of the butanol extract (BE) at doses of 8 and 16 mg/kg significantly (P<0.001) lowered the plasma glucose levels in diabetic rats after three hours of the administration. As a reference drug glibenclamide was used and showed, at a dose of 3 mg/kg, similar hypoglycemic effect like the tested extracts. Three kaempferol glucosides and one caffeoyl glucoside were isolated from the drug and were shown to be the main constituents in both extracts.

diabetes, hypoglycemic effect, Equisetum myriochaetum, streptozotocin, kaempferol glycosides

NCBI PubMed ID: 10967463
Publication DOI: 10.1016/s0378-8741(00)00218-x
Journal NLM ID: 7903310
Publisher: Limerick: Elsevier Sequoia
Institutions: Biological Department, Science School, National University of México UNAM, México, Mexico, Pharmaceutical Institute, Bonn, Germany, Metabolic Diseases Research Unit, National Medical Center, México, Mexico
Methods: 1H NMR, FAB-MS, biological assays, HPLC, extraction, CC, evaporation, flash chromatography

The flavonoids of Asplenium foreziense, A. fontanum subsp. fontanum and subsp. pseudofontanum, A. obovatum subsp. obovatum var. obovatum and var. protobillotii, A. obovatum subsp. lanceolatum, and A. incisum were isolated and identified for chemotaxonomic survey. A major constituent of all taxa was kaempferol 3-O-gentiobioside. As minor compounds, kaempferol 3,7-O-glycoside and/or kaempferol 3-O-glycoside were found in A. fontanum, A. obovatum and A. foreziense, and kaempferol 3-O-gentiobioside-4'-O-glucoside, kaempferol 3-O-glucoside and quercetin 3-O-diglucoside in A. incisum. It was suggested that A. foreziense, A. fontanum including subsp. pseudofontanum and A. obovatum including subsp. lanceolatum are not only morphologically but also chemotaxonomically related. The East Asian A. incisum was chemically and geographically different from these taxa.

Asplenium fontanum, Asplenium foreziense, Asplenium incisum, Asplenium obovatum, Aspleniaceae, kaempferol 3-O-gentiobioside, kaempferol 3-O-gentiobioside-4'-O-glucoside

NCBI PubMed ID: 10854741
Publication DOI: 10.1016/s0305-1978(99)00103-9
Journal NLM ID: 0430442
Publisher: Pergamon Press
Correspondence: iwashina@kahaku.go.jp
Institutions: Tsukuba Botanical Garden, National Science Museum, Tsukuba, Japan, Laboratorio de Arqueobotanica, Centro de Estudios Historicos, CSIC, Madrid, Spain, Departamento de Biologia Vegetal I, Facultad de Biologia, Universidad Complutense, Madrid, Spain, Laboratory of Pharmacognosy, Showa College of Pharmaceutical Sciences, Machida, Japan
Methods: 13C NMR, 1H NMR, FAB-MS, paper chromatography, HPLC, UV, extraction, CC, preparative paper chromatography, evaporation

Asplenium fontanum, Asplenium foreziense, Asplenium incisum, Asplenium obovatum, Aspleniaceae, kaempferol 3-O-gentiobioside, kaempferol 3-O-gentiobioside-4'-O-glucoside