Two new saikosaponins have been identified in the butanolic fraction of the ethanol extract of the roots of Bupleurum spinosum: 3β,16α,23,28-tetrahydroxyoleana-11,13(18)-dien-30-oic acid 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→3)-β-D-fucopyranoside, and 3β,16α,23,28,30-pentahydroxyoleana-11,13(18)-diene 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→3)-β-D-fucopyranoside. 3β,16β,23-trihydroxy-13,28-epoxyolean-11-ene 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→3)-β-D-fucopyranoside was also isolated, and this structure agreed with the one proposed for bupleuroside I, but their spectroscopic data have not been described until now. From the same fraction of the roots of Bupleurum gibraltaricum, the known compound buddlejasaponin IV has been isolated as the predominant component (90%). Structures were elucidated using spectroscopic analysis, specially 2D-NMR experiments.
Umbelliferae, Bupleurum spinosum, Bupleurum gibraltaricum, saikosaponins, bupleuroside I
NCBI PubMed ID: 11014258Publication DOI: 10.1016/s0031-9422(00)00177-1Journal NLM ID: 0151434Publisher: Elsevier
Correspondence: afbarre@goliat.ugr.es
Institutions: Departamento de Química Orgánica, Facultad Ciencias, Instituto de Bioteciología, Universidad de Granada, Spain, Laboratoire de Chimie Organique, Faculté des Sciences, Université Abdelmalek Essaadi, Tétouan, Morocco, Area de Química Orgánica, Facultad de Ciencias Experimentales, Universidad de Almería, Almería, Spain
Methods: 13C NMR, 1H NMR, NMR-2D, IR, FAB-MS, TLC, HPLC, UV, extraction, optical rotation measurement, CC, evaporation, HR-FAB-MS
Found