Three new steroidal saponins (1-3) were isolated from the fruits of Tribulus terrestris. Their structures were assigned by spectroscopic methods (IR, HRESIMS, 1D- and 2D-NMR) as 26-O-β-D-glucopyranosyl-(25S)-5β-furost-20(22)-en-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1-->2)-[α-L-rhamnopyranosyl- (1→4)]-β-D-glucopyranoside (1), 26-O-β-D-glucopyranosyl-(25S)-5β-furost-20(22)-en-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-galactopyranoside (2), and 25(S)-5β-spirostan-3β-ol-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-galactopyranoside (3). Compound 3 showed cytotoxicity against a human malignant melanoma cell line (SK-MEL).
cytotoxicity, glycosides, steroid, saponins, Tribulus terrestris
NCBI PubMed ID: 11141122Publication DOI: 10.1021/np000353bJournal NLM ID: 7906882Publisher: American Society of Pharmacognosy
Correspondence: Bedir E
; Khan IA
Institutions: Department of Pharmacognosy, School of Pharmacy, University of Mississippi, Mississippi, USA, Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, Missisippi, USA
Methods: 13C NMR, 1H NMR, NMR-2D, IR, TLC, ESI-MS, extraction, optical rotation measurement, CC, HR-ESI-MS, VLC
Found