Five steroidal saponins were isolated from the EtOH extract of Cestrum sendtenerianum (Solanaceae), as confirmed by detailed analysis of their 1H, 13C, and two-dimensional NMR spectral data, and by the results of hydrolytic cleavage. The saponins were revealed to contain three hydroxyl groups at the C-1β, C-2α, and C-3β positions in the spirostanol skeleton, and to bear a di- or triglycoside at C-3 as the common structural features. One of the compounds, a spirostanol triglycoside, showed weak cytotoxic activity on HL-60 human promyelocytic leukemia cells, with an IC50 value of 7.7 μg/ml.
cytotoxic activity, Solanaceae, spirostanol saponins, HL-60 cells, Cestrum sendtenerianum
NCBI PubMed ID: 11190386Publication DOI: 10.1016/s0031-9422(00)00109-6Journal NLM ID: 0151434Publisher: Elsevier
Correspondence: mimakiy@ps.toyaku.ac.jp
Institutions: Centro de Sanidade Animal, Instituto Biológico, São Paulo, Brazil, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
Methods: 13C NMR, 1H NMR, NMR-2D, inhibition studies, TLC, acid hydrolysis, HPLC, extraction, optical rotation measurement, CC, precipitation, evaporation, centrifugation, MTT, MPLC, HR-FAB-MS
Found