Administration of hydroxycoumarins to the cultured cells of Nicotiana tabacum caused the ladder fragmentation of genomic DNA of the cells and the secretion of the intracellular phytoalexin, scopoletin, into the culture medium, and also the exogenous hydroxycoumarins were transformed into their corresponding β-d-glucosides. The glucosylation seems to be a defense reaction of the cells for detoxification of toxic hydroxycoumarins, because their glucosides did not cause the DNA fragmentation.
glucosylation, detoxification, Nicotiana tabacum, cultured cells, DNA fragmentation, hydroxycoumarin, scopoletin, apoptosis-like cell death
Publication DOI: 10.1016/S1381-1177(00)00007-2Journal NLM ID: 9602616Publisher: Amsterdam: Elsevier
Correspondence: thirata@sci.hiroshima-u.ac.jp
Institutions: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan, Instrument Center for Chemical Analysis, Hiroshima University, Higashi-Hiroshima, Japan
Methods: 1H NMR, FAB-MS, HPLC, enzymatic digestion, cell growth, DNA extraction, precipitation, PTLC
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