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1. (Article ID: 319)
 
Manzoni M, Bergomi S, Rollini M, Cavazzoni V, Felisaz C, Marinoni C
Extracellular K5 polysaccharidic antigen recovery performed by different cell separation technologies
Biotechnology Letters 22(9) (2000) 759-766
 

The K5 polysaccharidic antigen obtainable from a strain of Escherichia coli is the non-sulphated precursor in heparin biosynthesis; K5 is composed by two components, 16 000 and 1500 Da, their ratio depending on the fermentation conditions. In this study an assessment was made of how various cell-culture filtrate separation technologies affected the components themselves and their ratio. A dynamic membrane filtration technology, a tubular ceramic module for tangential flow microfiltration and the standard discontinuous centrifugation were comparatively employed to perform the separation. Experiments carried out on E. coli cultures containing the two components in different ratios showed that the DMF system is a suitable technology for K5 isolation.

antigen, cell, Escherichia coli, capsular polysaccharide, extracellular, dynamic microfiltration technology, fermentation, recovery, separation

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2. (Article ID: 481)
 
Crich D, Yao Q
Benzylidene Acetal Fragmentation Route to 6-Deoxy Sugars: Direct Reductive Cleavage in the Presence of Ether Protecting Groups, Permitting the Efficient, Highly Stereocontrolled Synthesis of β-d-Rhamnosides from d-Mannosyl Glycosyl Donors. Total Synthesis of α-D-Gal-(1→3)-α-D-Rha-(1→3)-β-D-Rha-(1→4)-β-D-Glu-OMe, the Repeating Unit of the Antigenic Lipopolysaccharide from Escherichia hermannii ATCC 33650 and 33652
Journal of the American Chemical Society 126(26) (2004) 8232-8236
 

An approach to the stereocontrolled synthesis of β-D-rhamnopyranosides is described in which 2,3-O-benzyl or related 4,6-O-[α-(2-(2-iodophenyl)ethylthiocarbonyl)benzylidene]-mannosyl thioglycosides are first used to introduce the β-D-mannopyranoside linkage in high yield and stereoselectivity. Following glycosylation, treatment with tributyltin hydride in toluene at reflux brings about reductive radical fragmentation directly to the 6-deoxy sugar in high yield. A variation of these donors bearing a carboxylated donor on O3 is a highly alpha-selective mannosyl and, after radical fragmentation, α-D-rhamnosyl donor. Using this stereoselective glycosylation/radical-fragmentation approach, a concise synthesis of the title tetrasaccharide is realized in which both the β-D- and α-D-rhamnopyranosyl units are obtained in a single step by a double radical fragmentation of the modified benzylidene acetals.

Lipopolysaccharide, repeating unit, glycosylation, Escherichia hermannii, stereoselective

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