The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: where R=α-D-Galp-(1→4)-β-D-GalpNAc-(1→ or α-D-Galp-(1→ (approx. ratio 4:3). Comparative CE-MS analysis of A. salmonicida subsp. salmonicida core oligosaccharides from strains A449, 80204-1 and an in vivo rough isolate confirmed that the structure of the core oligosaccharide was conserved among different isolates of A. salmonicida.

Lipopolysaccharide, NMR, core oligosaccharide, Aeromonas salmonicida, CE-MS

• Compound ID: 7012
  

  a-D-Galp-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-+ | a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdo | L-gro-a-D-manHepp-(1-6)-+

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G47828HG, GlycomeDB:27907
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449, Aeromonas salmonicida 80204-1
  CSDB ID 20229 (all data & tools)
• Compound ID: 7017
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ P-4)-+ P-4)-+ | | | a-D-Galp-(1-4)-b-D-GalpN-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P | L-gro-a-D-manHepp-(1-6)-+

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Trivial name: glycoform-OH-1
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GlycomeDB:28151
  
   
  
  Aeromonas salmonicida A449
  CSDB ID 20814 (all data & tools)
• Compound ID: 7013
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ | a-D-Galp-(1-4)-b-D-GalpNAc-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdo | L-gro-a-D-manHepp-(1-6)-+

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G97567DV, GlycomeDB:28147
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20810 (all data & tools)
• Compound ID: 7014
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ | a-D-Galp-(1-4)-b-D-GalpN-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdo | L-gro-a-D-manHepp-(1-6)-+

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G32344KH, GlycomeDB:28148
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20811 (all data & tools)
• Compound ID: 7015
  

  a-D-Galp-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-+ P-4)-+ P-4)-+ | | | a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P | L-gro-a-D-manHepp-(1-6)-+

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GlycomeDB:28149
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20812 (all data & tools)
• Compound ID: 7016
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ P-4)-+ P-4)-+ | | | a-D-Galp-(1-4)-b-D-GalpNAc-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P | L-gro-a-D-manHepp-(1-6)-+

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GlycomeDB:28150
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20813 (all data & tools)
• Compound ID: 7018
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ | b-D-Galp-(1-4)-+ | P-4)-+ | | | D-gro-a-D-manHepp-(1-6)-D-gro-a-D-manHepp-(1-6)-a-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ | L-gro-a-D-manHepp-(1-6)-+

  Show graphically

  Show legend Show as text

  Structure type: oligomer
  Aglycon: lipid A
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G76206IA, GlycomeDB:28152
  
   
  
  Aeromonas salmonicida 438
  CSDB ID 20815 (all data & tools)

The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating units with one or two different monosaccharides have been described. Functional genomic analysis of this wbsalmo region is mostly in agreement with the LPS O-antigen structure of acetylated l-rhamnose (Rha), d-glucose (Glc), and 2-amino-2-deoxy-d-mannose (ManN). Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains). Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer. A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens.

lipopolysaccharide O-antigen, 2-amino-2-deoxy-D-mannose, genomics, Aeromonas salmonicida, subspecies, Proteomics, A-surface layer

Publication DOI: 10.3390/md13063791
Journal NLM ID: 101213729
Publisher: Basel, Switzerland: Molecular Diversity Preservation International
Correspondence: elenademendozahotmail.com (E.M.); jtomasub.edu (J.M.T.); rcanalsgmail.com
Institutions: Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK, Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona 08071, Spain
Methods: methylation, PCR, SDS-PAGE, sugar analysis, DNA techniques, GLC, mild acid hydrolysis, de-O-acetylation, genetic methods, bioinformatic analysis, LC-ESI-MS
 

• Compound ID: 7334
  

  a-D-Glcp-(1-3)-+ | -3)-b-D-ManpNAc-(1-4)-a-L-Rhap2Ac-(1-

  Show graphically

  Show legend Show as text

  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide, O-antigen
  Reference(s) to other database(s): GTC:G26810WR, GlycomeDB:27630
  
   
  
  Aeromonas salmonicida ssp. salmonicida A450, Aeromonas salmonicida ssp. salmonicida A449, Aeromonas salmonicida ssp. salmonicida 80204
  CSDB ID 30639 (all data & tools)