The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: where R=α-D-Galp-(1→4)-β-D-GalpNAc-(1→ or α-D-Galp-(1→ (approx. ratio 4:3). Comparative CE-MS analysis of A. salmonicida subsp. salmonicida core oligosaccharides from strains A449, 80204-1 and an in vivo rough isolate confirmed that the structure of the core oligosaccharide was conserved among different isolates of A. salmonicida.

Lipopolysaccharide, NMR, core oligosaccharide, Aeromonas salmonicida, CE-MS

• Compound ID: 7012
  

  a-D-Galp-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-+ | a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdo | L-gro-a-D-manHepp-(1-6)-+

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  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G47828HG, GlycomeDB:27907
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449, Aeromonas salmonicida 80204-1
  CSDB ID 20229 (all data & tools)
• Compound ID: 7017
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ P-4)-+ P-4)-+ | | | a-D-Galp-(1-4)-b-D-GalpN-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P | L-gro-a-D-manHepp-(1-6)-+

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  Structure type: oligomer
  Trivial name: glycoform-OH-1
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GlycomeDB:28151
  
   
  
  Aeromonas salmonicida A449
  CSDB ID 20814 (all data & tools)
• Compound ID: 7013
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ | a-D-Galp-(1-4)-b-D-GalpNAc-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdo | L-gro-a-D-manHepp-(1-6)-+

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  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G97567DV, GlycomeDB:28147
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20810 (all data & tools)
• Compound ID: 7014
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ | a-D-Galp-(1-4)-b-D-GalpN-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdo | L-gro-a-D-manHepp-(1-6)-+

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  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G32344KH, GlycomeDB:28148
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20811 (all data & tools)
• Compound ID: 7015
  

  a-D-Galp-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-+ P-4)-+ P-4)-+ | | | a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P | L-gro-a-D-manHepp-(1-6)-+

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  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GlycomeDB:28149
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20812 (all data & tools)
• Compound ID: 7016
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ P-4)-+ P-4)-+ | | | a-D-Galp-(1-4)-b-D-GalpNAc-(1-4)-L-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P | L-gro-a-D-manHepp-(1-6)-+

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  Structure type: oligomer
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GlycomeDB:28150
  
   
  
  Aeromonas salmonicida ssp. salmonicida A449
  CSDB ID 20813 (all data & tools)
• Compound ID: 7018
  

  a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+ | b-D-Galp-(1-4)-+ | P-4)-+ | | | D-gro-a-D-manHepp-(1-6)-D-gro-a-D-manHepp-(1-6)-a-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/ | L-gro-a-D-manHepp-(1-6)-+

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  Structure type: oligomer
  Aglycon: lipid A
  Compound class: core oligosaccharide
  Reference(s) to other database(s): GTC:G76206IA, GlycomeDB:28152
  
   
  
  Aeromonas salmonicida 438
  CSDB ID 20815 (all data & tools)