Bioactive compounds were produced from natural glucosinolates, secondary plant metabolites, using myrosinase (thioglucoside glucohydrolase EC 3.2.3.1) isolated from ripe seeds of Sinapis alba. The enzyme was immobilized on granular nylon 6.6 with the crosslinking technique. Immobilized myrosinase displayed extraordinary operational and storage stability. Using a small thermostatted continuous packed-bed bioreactor, the enzyme activity was unchanged after 15 days of continuous use at 37°C and after >1 year of storage at room temperature. The bioreactor was particularly efficient in producing pure isothiocyanates, but it was less efficient for pure nitrile production.
immobilization, bioreactor, myrosinase, glucosinolates, isothiocyanates, nitriles
NCBI PubMed ID: 10799991Publication DOI: 10.1002/(sici)1097-0290(20000620)68:6<660::aid-bit9>3.0.co;2-lJournal NLM ID: 7502021Publisher: New York: Wiley-VCH
Correspondence: sandro.palmieri@iol.it
Institutions: Istituto Sperimentale per le Colture Industriali of Italian Ministry of Agricultural and Forestry Politics, Bologna, Italy
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