Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 32093600Publication DOI: 10.1134/S000629792002011XJournal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: yknirel
gmail.com
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4001, Australia, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701, Russia
Capsular polysaccharide (CPS), isolated from Acinetobacter baumannii LUH5549 carrying the KL32 capsule biosynthesis gene cluster, was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy. The K32 CPS was found to be composed of branched pentasaccharide repeats (K units) containing two residues of β-D-GalpNAc and one residue of β-D-GlcpA (β-D-glucuronic acid) in the main chain and one residue each of β-D-Glcp and α-D-GlcpNAc in the disaccharide side chain. Consistent with the established CPS structure, the KL32 gene cluster includes genes for a UDP-glucose 6-dehydrogenase (Ugd3) responsible for D-GlcA synthesis and four glycosyltransferases that were assigned to specific linkages. Genes encoding an acetyltransferase and an unknown protein product were not involved in CPS biosynthesis. Whilst the KL32 gene cluster has previously been found in the global clone 2 (GC2) lineage, LUH5549 belongs to the sequence type ST354, thus demonstrating horizontal gene transfer between these lineages.
Acinetobacter baumannii, polysaccharide structure, capsule, glucuronic acid, K locus
Structure type: polymer chemical repeating unit
Location inside paper: p.244, fig.3, CPS
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137473,IEDB_140630,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_153510,IEDB_423153,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, acid hydrolysis, GLC, Smith degradation, GPC, bioinformatic analysis
Enzymes that release or process the structure: Gtr67, Gtr68, Gtr69, Gtr70, Wzy(K32)[ItrA2]
Related record ID(s): 3279
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G23026SQ
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,2 Ac 175.3-175.5 23.3-24.0
3,4,4,6 bDGlcp 104.0 74.5 77.2 71.2 77.2 62.4
3,4,4,2 Ac 175.3-175.5 23.3-24.0
3,4,4 aDGlcpN 98.3 55.3 71.9 70.9 72.0 69.3
3,4 bDGalpN 102.9 52.8 79.0 74.9 77.0 61.6
3 bDGlcpA 105.3 73.5 75.2 81.2 74.8 172.1
2 Ac 175.3-175.5 23.3-24.0
bDGalpN 104.1 52.5 81.3 69.2 75.9 62.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,2 Ac - 1.96-2.06
3,4,4,6 bDGlcp 4.47 3.35 3.49 3.40 3.43 3.72-3.91
3,4,4,2 Ac - 1.96-2.06
3,4,4 aDGlcpN 4.86 3.90 3.87 3.71 4.34 4.06-4.32
3,4 bDGalpN 4.53 4.06 3.84 4.22 3.73 3.64-3.68
3 bDGlcpA 4.58 3.40 3.64 3.84 4.01 -
2 Ac - 1.96-2.06
bDGalpN 4.54 3.83 3.89 4.06 3.59 3.74-3.82
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,2 Ac 23.3-24.0/1.96-2.06
3,4,4,6 bDGlcp 104.0/4.47 74.5/3.35 77.2/3.49 71.2/3.40 77.2/3.43 62.4/3.72-3.91
3,4,4,2 Ac 23.3-24.0/1.96-2.06
3,4,4 aDGlcpN 98.3/4.86 55.3/3.90 71.9/3.87 70.9/3.71 72.0/4.34 69.3/4.06-4.32
3,4 bDGalpN 102.9/4.53 52.8/4.06 79.0/3.84 74.9/4.22 77.0/3.73 61.6/3.64-3.68
3 bDGlcpA 105.3/4.58 73.5/3.40 75.2/3.64 81.2/3.84 74.8/4.01
2 Ac 23.3-24.0/1.96-2.06
bDGalpN 104.1/4.54 52.5/3.83 81.3/3.89 69.2/4.06 75.9/3.59 62.6/3.74-3.82
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,2 | Ac |
| 1.96
2.06 | |
| 3,4,4,6 | bDGlcp | 4.47 | 3.35 | 3.49 | 3.40 | 3.43 | 3.72
3.91 |
| 3,4,4,2 | Ac |
| 1.96
2.06 | |
| 3,4,4 | aDGlcpN | 4.86 | 3.90 | 3.87 | 3.71 | 4.34 | 4.06
4.32 |
| 3,4 | bDGalpN | 4.53 | 4.06 | 3.84 | 4.22 | 3.73 | 3.64
3.68 |
| 3 | bDGlcpA | 4.58 | 3.40 | 3.64 | 3.84 | 4.01 |
|
| 2 | Ac |
| 1.96
2.06 | |
| | bDGalpN | 4.54 | 3.83 | 3.89 | 4.06 | 3.59 | 3.74
3.82 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,2 | Ac | 175.3
175.5 | 23.3
24.0 | |
| 3,4,4,6 | bDGlcp | 104.0 | 74.5 | 77.2 | 71.2 | 77.2 | 62.4 |
| 3,4,4,2 | Ac | 175.3
175.5 | 23.3
24.0 | |
| 3,4,4 | aDGlcpN | 98.3 | 55.3 | 71.9 | 70.9 | 72.0 | 69.3 |
| 3,4 | bDGalpN | 102.9 | 52.8 | 79.0 | 74.9 | 77.0 | 61.6 |
| 3 | bDGlcpA | 105.3 | 73.5 | 75.2 | 81.2 | 74.8 | 172.1 |
| 2 | Ac | 175.3
175.5 | 23.3
24.0 | |
| | bDGalpN | 104.1 | 52.5 | 81.3 | 69.2 | 75.9 | 62.6 |
|
There is only one chemically distinct structure:
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