Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
NCBI PubMed ID: 32252300Publication DOI: 10.3390/ijms21072465Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: michelle.kilcoyne
nuigalway.ie
Institutions: Carbohydrate Signalling Group, Discipline of Microbiology, National University of Ireland Galway, H91 TK33 Galway, Ireland, Infectious Disease Laboratory, Discipline of Microbiology, National University of Ireland Galway, H91 TK33 Galway, Ireland, Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, H91 TK33 Galway, Ireland, Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
The biofilm component poly-N-acetylglucosamine (PNAG) is an important virulence determinant in medical-device-related infections caused by ESKAPE group pathogens including Gram-positive Staphylococcus aureus and Gram-negative Acinetobacter baumannii. PNAG presentation on bacterial cell surfaces and its accessibility for host interactions are not fully understood. We employed a lectin microarray to examine PNAG surface presentation and interactions on methicillin-sensitive (MSSA) and methicillin-resistant S. aureus (MRSA) and a clinical A. baumannii isolate. Purified PNAG bound to wheatgerm agglutinin (WGA) and succinylated WGA (sWGA) lectins only. PNAG was the main accessible surface component on MSSA but was relatively inaccessible on the A. baumannii surface, where it modulated the presentation of other surface molecules. Carbohydrate microarrays demonstrated similar specificities of S. aureus and A. baumannii for their most intensely binding carbohydrates, including 3' and 6'sialyllactose, but differences in moderately binding ligands, including blood groups A and B. An N-acetylglucosamine-binding lectin function which binds to PNAG identified on the A. baumannii cell surface may contribute to biofilm structure and PNAG surface presentation on A. baumannii. Overall, these data indicated differences in PNAG presentation and accessibility for interactions on Gram-positive and Gram-negative cell surfaces which may play an important role in biofilm-mediated pathogenesis
polysaccharide, Acinetobacter baumannii, Staphylococcus aureus, lectin, PNAG, Biofilm, bacterial adhesins, glycomics microarrays, poly-N-acetylglucosamine
Structure type: polymer chemical repeating unit
Location inside paper: Fig.1a
Trivial name: PNAG
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531
Methods: biofilm assays, PNAG assay on bacterial cell surface, lectin and carbohydrate microarray construction
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G17753QF
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error