High-performance liquid chromatography–electrospray ionization mass spectrometry has been applied to analyze the flavonoids of Huangqi, the roots of Astagalus mongholicus and A. membranaceus. Eight flavonoids were identified as calycosin-7-O-β-D-glucoside, calycosin-7-O-β-D-glucoside-6″-O-malonate (2), ononin, (6aR,11aR)-3-hydroxy-9,10-dimethoxypterocarpan-3-O-β-D-glucoside, calycosin, (3R)-7,2′-dihydroxy-3′,4′-dimethoxyisoflavan-7-O-β-D-glucoside, formononetin-7-O-β-D-glucoside-6″-O-malonate and formononetin by direct comparison with the isolated standards from Huangqi. The existence of (6aR,11aR)-3-hydroxy-9,10-dimethoxypterocarpan, (3R)-7,2′-dihydroxy-3′,4′-dimethoxyisoflavan, astrapterocarpanglucoside-6′-O-malonate and astraisoflavanglucoside-6′-O-malonate was detected. This is the first report of flavonoid glycoside malonates in these two Astragalus species, and malonate 2 is a structurally completely identified new compound.
plant materials, Astragalus membranaceus, Astragalus mongholicus
NCBI PubMed ID: 10823504Publication DOI: 10.1016/s0021-9673(00)00149-7Journal NLM ID: 9318488Publisher: Amsterdam; New York: Elsevier
Correspondence: longze.lin@amtbotanicals.com
Institutions: Research Laboratory of Natural Products Chemistry, A.M. Todd Botanicals, Eugene, USA, Program for Collaborative Research in the Pharmaceutical Sciences, College of Pharmacy, The University of Illinois at Chicago, Chicago, IL, USA
Methods: LC-ESI-MS
Found