Qualitative and quantitative analyses of endogenous jasmonoids were done by liquid chromatography/ selected ion monitoring (LC-SIM) using deuterium-labeled compounds as internal standards. To prove the practicality of this way of analyzing the contents of endogenous jasmonoids in plants, the method was used for estimating jasmonoids in potato plants.
Solanum tuberosum L., potato micro-tuber inducing stimuli, jasmonoids, liquid chromatography/selected ion monitoring (LC-SIM)
NCBI PubMed ID: 11193405Publication DOI: 10.1271/bbb.64.2380Journal NLM ID: 9205717Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Correspondence: yosihara@chem.agr.hokudai.ac.jp
Institutions: Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Japan
Methods: 1H NMR, extraction, isotopic labeling, FD-MS, HR-EI-MS