Two saponins and a sapogenin were isolated from the leaves of Ilex brevicuspis. Their structures were established by means of spectroscopic methods as brevicuspisaponin 1 (3-O-α-L-arabinopyranosyl-20(S)-19α,24-dihydroxyursolic acid), brevicuspisaponin 2 (3-O-α-L-arabinopyranosyl-20(S)-19α,23,24-trihydroxyursolic acid) and 23-methylester of 20(S)-3β,19α,24-trihydroxyurs-12-en-23,28-dioic acid.
Ilex brevicuspis; Aquifoliaceae; saponin; sapogenin; brevicuspisaponin 1 and 2, 20(S)-rotungenic acid, 20(S)-rotundioic acid; ursolic acid
NCBI PubMed ID: 10820800Publication DOI: 10.1016/s0031-9422(99)00517-8Journal NLM ID: 0151434Publisher: Elsevier
Correspondence: schenkel@farmacia.ufrgs.br
Institutions: Kekulé-Institut für Organische Chemie und Biochemie der Rheinischen Friedrich-Wilhelms-Universität, Bonn, Germany, Laboratoire de Chimie Thérapeutique, URA 1310 du CNRS, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris V, Paris, France, Faculdade de Farmácia, UFRGS, Porto Alegre, Brazil
Methods: 13C NMR, 1H NMR, NMR-2D, TLC, ESI-MS, acid hydrolysis, HPLC, extraction, CC, HR-ESI-MS, evaporation, centrifugation
Found