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1. (Article ID: 3319)
 
Wittkowski M, Mittelstadt J, Brandau S, Reiling N, Lindner B, Torrelles J, Brennan PJ, Holst O
Capsular arabinomannans from Mycobacterium avium with morphotype specific structural differences but identical biological activity
Journal of Biological Chemistry 282(26) (2007) 19103-19112
 

The capsules of two colony-morphotypes of Mycobacterium avium strain 2151 were investigated, i.e. of the virulent smooth-transparent (SmT) and the non-virulent smooth-opaque (SmO) type. From both morphotypes we separated a non-acylated arabinomannan (AM) from an acylated polysaccharide fraction by affinity chromatography, of which the AMs were structurally characterised. The AMs from the virulent morphotype in contrast to that from the non-virulent form possessed a larger mannan chain and a shorter arabinan chain. Incubation of murine bone marrow derived macrophages and human dendritic cells showed that the acylated polysaccharide fractions were potent inducers of TNF , IL 12 and IL-10, compared to non-acylated AMs which only led to a marginal cytokine release. Further in vitro experiments showed that both, the acylated polysaccharide fractions as well as the non-acylated AMs were able to induce in vitro anti-tumor cytotoxicity of human peripheral blood mononuclear cells. Thus, morphotype specific structural differences in capsular AMs of M. avium do not correlate with biological activity, however, their acylation is a prerequisite for effective stimulation of murine macrophages and human dendritic cells

biological activity, Mycobacterium avium, cells, virulent, macrophages, capsules, arabinomannan

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