An API-LC/MS/MS method was developed for the identification of the medicinal herb Podophyllum emodi based on the profile of its aryltetrahydronapthalene and related lignan marker compounds. This was done by matching the structural information from the tandem mass spectrometric data with those lignan marker compounds already reported for the herb. The method could be employed in the absence of reference standards for the markers and was particularly useful in view of the scarcity of supply of these chemical standards. It has been used successfully to differentiate Podophyllum emodi from two commonly used medicinal herbs of a different genus but having similar appearance, Radix clematidis and Radix gentiana, as well as a closely related herb, Podophyllum peltatum.
Podophyllum emodi; API-LC/MS/MS; marker compound proļ¬le
NCBI PubMed ID: 11114081Publication DOI: 10.1002/1096-9888(200011)35:11<1246::AID-JMS55>3.0.CO;2-LJournal NLM ID: 9504818Publisher: Chichester, UK: Wiley
Institutions: National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China, Government Laboratory, Homantin, Hong Kong, China
Methods: HPLC, extraction, API-LC/MS/MS
Found