Six oleanane-type saponins, along with two known triterpene saponins, were isolated from the leaves of Gymnema sylvestre. The structures of the oleanane triterpene glycosides were characterized as longispinogenin 3-O-b-D-glucuronopyranoside, 21b-benzoylsitakisogenin 3-O-b-D-glucuronopyranoside, 3-O-b-D-glucopyranosyl(1 4 6)-b-D-glucopyranosyl oleanolic acid 28-O-b-D-glucopyranosyl ester, oleanolic acid 3-O-b-D-xylopyranosyl(1 4 6)-b-D-glucopyranosyl(1 4 6)-b-D-glucopyranoside, 3-O-b-D-xylopyranosyl(1 4 6)-b-D-glucopyranosyl(1 4 6)-b-D-glucopyranosyl oleanolic acid 28-O-b-D-glucopyranosyl ester and 3-O-b-D-glucopyranosyl(1 46)-b-D-glucopyranosyl oleanolic acid 28-b-D-glucopyranosyl(1 46)-b-D-glucopyranosyl ester on the basis of hydrolysis and spectral evidence, including 1D- and 2D-NMR (TOCSY, ROESY, HMQC and HMBC) and FABMS analyses
Gymnema sylvestre; Asclepiadaceae; oleanane saponins
NCBI PubMed ID: 10820799Publication DOI: 10.1016/s0031-9422(99)00483-5Journal NLM ID: 0151434Publisher: Elsevier
Correspondence: chect@cuhk.edu.hk
Institutions: Department of Phytochemistry, China Pharmaceutical University, Nanjing, China, School of Chinese Medicine, the Chinese University of Hong Kong, Shatin, Hong Kong, China, Department of Chemistry, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China
Methods: 13C NMR, 1H NMR, IR, TLC, HPLC, UV, extraction, optical rotation measurement, melting point determination, HR-FAB-MS
Found