A new bisdesmosidic spirostanol saponin, along with three known saponins, were isolated from Agave americana (Agavaceae). The structure of the new saponin was elucidated as (25R)-3β,6α-dihydroxy-5α-spirostan-12-one 3,6-di-O-β-D-glucopyranoside. Among the isolated saponins, hecogenin tetraglycoside showed cytotoxic activity against HL-60 human promyelocytic leukemia cells with an IC50 value of 4.3 μg/mL.
cytotoxic activity, spirostanol saponin, HL-60 leukemia cells, Agave americana
NCBI PubMed ID: 10865469Publication DOI: 10.1055/s-2000-8546Journal NLM ID: 0066751Publisher: George Thieme
Correspondence: mimakiy@ps.toyaku.ac.jp
Institutions: School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
Methods: 13C NMR, 1H NMR, NMR-2D, FAB-MS, TLC, ESI-MS, HPLC, extraction, CC
Found