The saponin fraction from the fresh leaves of Euptelea polyandra SIEB. et ZUCC. was found to exhibit potent gastroprotective activity. Fourteen new nortriterpene saponins called eupteleasaponins were isolated from the saponin fraction with gastroprotective activity. The structures of eupteleasaponins I, II, III, IV, V, and V acetate were determined on the basis of chemical and physicochemical evidence as 3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranosyl(1→3)-β-D-xylopyranosylakebonoic acid 28-O-β-D-glucopyranosyl ester, 3-O-α-L-rhamnopyranosyl(1→2)-[α-L-rhamnopyranosyl(1→4)]-β-D-glucopyranosyl(1→3)-β-D-xylopyranosylakebonoic acid 28-O-β-D-glucopyranosyl ester, 3-O-α-L-rhamnopyranosyl(1→2)-[α-L-arabinopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)]-β-D-glucopyranosyl(1→3)-β-D-xylopyranosylakebonoic acid 28-O-β-D-glucopyranosyl ester, 3-O-α-L-rhamnopyranosyl(1→2)-[α-L-arabinopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)]-β-D-glucopyranosyl(1→3)-β-D-xylopyranosylakebonoic acid, 3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranosyl(1→3)-β-D-xylopyranosyleupteleogenin, and 3-O-α-L-rhamnopyranosyl(1→2)-6"-O-acetyl-β-D-glucopyranosyl(1→3)-β-D-xylopyranosyleupteleogenin
gastroprotective activity, Euptelea polyandra, eupteleasaponin, akebonoic acid, eupteleogenin, Eupteleaceae
NCBI PubMed ID: 10923838Publication DOI: 10.1248/cpb.48.1045Journal NLM ID: 0377775Publisher: Pharmaceutical Society Of Japan
Correspondence: Yoshikawa M
Institutions: Kyoto Pharmaceutical University, Kyoto, Japan
Methods: 13C NMR, 1H NMR, NMR-2D, TLC, ESI-MS, acid hydrolysis, HPLC, extraction, CC, HR-ESI-MS, evaporation, centrifugation
Found