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1. (Article ID: 3385)
Kuebler-Kielb J, Vinogradov E, Chu C, Schneerson R
O-Acetylation in the O-specific polysaccharide isolated from Shigella flexneri serotype 2a
Carbohydrate Research 342(3-4) (2007)
643-647
Shigella flexneri causes diarrheal diseases especially in infants and children in developing countries. Modifications of the lipopolysaccharide (LPS) molecule, like bacteriophage-mediated glucosylation and acetylation of the O-specific chain (O-SP), are important for the LPS antigenicity and consequently for the immunogenicity of the polysaccharide-based vaccines against shigellosis. Here, we report the degree of O-acetylation and the localisation of O-acetyl groups and side-chain glucose substitution in the O-SP (scheme) in different preparations of S. flexneri type 2a LPS
LPS, O-acetylation, Shigella, Flexneri, O-specific chain
NCBI PubMed ID: 17087926Publication DOI: 10.1016/j.carres.2006.09.017Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: kielbj
mail.nih.gov
Institutions: National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA
Methods: 13C NMR, 1H NMR, NMR-2D, mild acid hydrolysis, alkaline hydrolysis
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