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1. (Article ID: 3389)
 
Kocharova NA, Ovchinnikova OG, Torzewska A, Shashkov AS, Knirel YA, Rozalski A
The structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O36 containing 3-deoxy-D-manno-oct-2-ulosonic acid
Carbohydrate Research 342(3-4) (2007) 665-670
 

An oligosaccharide that corresponds to the repeating unit of the O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O36. Structural studies of the oligosaccharide and O-deacylated lipopolysaccharide were performed using sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, and H-detected 1H,13C HSQC and HMBC experiments. It was found that the O-polysaccharide is built up of linear trisaccharide repeating units containing 2-acetamido-2-deoxyglucose, 6-deoxy-L-talose (L-6dTal), and 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) and has the following structure.

Lipopolysaccharide, structure, O-antigen, Kdo, Providencia alcalifaciens, 6-deoxy-L-talose, polysaccharide structure

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