The galactose-rich polysaccharide (GPS) in the cell wall of the Gram-positive bacterium Renibacterium salmoninarum, the causative agent in of bacterial kidney disease (BKD) of salmonids, has been studied by sugar and methylation analysis, partial acid hydrolysis, Smith degradation, FABMS, and 1H and 13C NMR spectroscopy. The data show that the GPS has a heptasaccharide repeating unit with the following structure: α-D-Rhap-(1→3)-α-L-FucpNAc-(1→)-β-D-GlcpNAc 1 decreases 2 →3)-β-D-Galf-(1→6)-β-D-Galf-(1→3)-β-D-Galf-(1→6)-β-D-Galf-(1→.
NMR, structure, cell wall polysaccharide, Renibacterium salmoninarum
NCBI PubMed ID: 9691455Publication DOI: 10.1016/s0008-6215(97)10071-4Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Marine Biochemistry, The Norwegian College of Fishery Science, University of Tromsø, N-9037 Tromsø, Norway, Department of Chemistry, Swedish University of Agricultural Sciences, Box 7015, S-750 07 Uppsala, Sweden
Methods: 13C NMR, 1H NMR, methylation, FAB-MS, partial acid hydrolysis, Smith degradation
Found