Show legend Show as text

Structure type: polymer chemical repeating unit
Trivial name: Beijeran, deacetylated beijeran
Compound class: EPS, O-polysaccharide, glucorhamnogalacturonan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

• Article ID: 14
  Bian W, Chandrasekaran R, Ogawa K "X-ray structure analysis of the sodium salt of beijeran" - Carbohydrate Research 337(4) (2002) 305-314
  

  The three-dimensional structure of the sodium salt of beijeran has been determined by X-ray fiber diffraction analysis. The acidic polysaccharide forms an extended twofold helix. Two chains are nestled tightly in a monoclinic unit cell of dimensions a=12.72, b=11.41, c (fiber axis)=24.62 A and γ=123.7 degree in an antiparallel fashion. In the crystalline lattice, helices are stacked tightly to form a thick sheet along the vertical plane passing through the short diagonal of the basal net. Adjacent sheets associate via a network of sodium ions and water molecules embedded between them. The morphology of sodium beijeran in the solid state is consistent with its observed rheological properties.

  structure, polysaccharide, analysis, carbohydrate, cell, chain, food, helix, ion, molecule, Research, salt, water, X-ray, acidic, acidic polysaccharide, crystalline, degree, form, lattice, morphology, network, property, rheological, solid, solid state

  NCBI PubMed ID: 11841811
  Publication DOI: 10.1016/s0008-6215(01)00277-4
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: chandra@purdue.edu
  Institutions: Whistler Center for Carbohydrate Research, 1160 Food Science Building, Purdue University, 47907-1160, West Lafayette, IN, USA
  Methods: X-ray
  
   
  
  Azotobacter beijerinckii YNM1
  CSDB ID 1615 (all data & tools)
• Article ID: 4437
  Ogawa K, Yui T, Nakata K, Kakuta M, Misaki A "X-Ray study of beijeran sodium salts, a new galacturonic acid-containing exo-polysaccharide" - Carbohydrate Research 300(1) (1997) 41-45
  

  X-Ray fiber diffraction patterns were obtained from oriented films of sodium salts of a new uronic acid-containing polysaccharide (beijeran) both in its native, poly [→3)-α-D-GalA-(1→3)-β-L-Rha-(1→3)-α-D-Glc-O6Ac-(1→], and deacetylated forms. Initially the stretched films of both polysaccharides were amorphous, but the crystallinity was much improved by annealing at high temperature. The deacetylated specimen had higher crystallinity than the native. Both films showed similar X-ray fiber patterns indicating that these polysaccharides had similar unit cell dimensions and that the O-acetyl groups in the native beijeran chain did not disturb the regular array in the crystal having space group P21. All the visible reflections could be indexed in terms of a monoclinic unit cell with dimensions a = 1.277, b = 1.611, c (fiber axis) = 2.437 nm, and γ = 96.79 degrees. The fiber axis length and the presence of (002) and (006) reflections indicated that the conformation was made up of two trisaccharide residues, in an extended two-fold helix.

  Beijeran, Chain conformation, Fiber diffraction pattern, Unit cell parameters

  NCBI PubMed ID: 9203333
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Research Institute for Advanced Science and Technology, Osaka Prefecture University, Japan
  Methods: X-ray
  
   
  
  Azotobacter beijerinckii
  CSDB ID 28852 (all data & tools)
• Article ID: 5294
  Ogawa K, Yui T, Nakata K, Nitta Y, Kakuta M, Misaki A "Chain conformation of deacetylated beijeran calcium salt" - Bioscience, Biotechnology, and Biochemistry 60(3) (1996) 551-553
  

  A well-defined X-ray fiber diffraction pattern was obtained from a stretched film of the calcium salt of a new uronic acid containing polysaccharide designated as beijeran in the deacetylated form, poly[→3)-α-D-GalUA-(1→3)-β-L-Rham-(1→3)-α-D-Glc-(1→]. The oriented film showed no diffraction spots, indicating it to be amorphous. However, when annealed at high temperature, the film exhibited high crystallinity. All the visible reflections could be indexed in terms of a monoclinic unit cell with the following dimensions: a = 1.297; b = 1.676; c (fiber axis) = 2.509 nm; and γ = 106.50°. The length of the fiber axis and the absence of meridional reflections at any odd layer line indicate that an extended two-fold helical conformation was made up of two trisaccharide residues.

  Chain conformation, Unit cell parameters, deacetylated beijeran calcium salt, X-ray fiber pattern

  NCBI PubMed ID: 8901123
  Publication DOI: 10.1271/bbb.60.551
  Journal NLM ID: 9205717
  Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  Institutions: Research Institute of Advanced Science and Technology, Osaka Prefecture University, Osaka, Japan, Faculty of Engineering, Osaka Prefecture University, Miyazaki, Japan, College of Agriculture, Osaka Prefecture University, Osaka, Japan, Kanan Women’s University, Kobe, Japan
  Methods: deacetylation, X-ray
  
   
  
  Azotobacter beijerinckii TNM 1
  CSDB ID 44216 (all data & tools)

Show legend Show as text

Structure type: polymer chemical repeating unit
Trivial name: beijeran
Compound class: EPS, O-polysaccharide, glucorhamnogalacturonan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

• Article ID: 4437
  Ogawa K, Yui T, Nakata K, Kakuta M, Misaki A "X-Ray study of beijeran sodium salts, a new galacturonic acid-containing exo-polysaccharide" - Carbohydrate Research 300(1) (1997) 41-45
  

  X-Ray fiber diffraction patterns were obtained from oriented films of sodium salts of a new uronic acid-containing polysaccharide (beijeran) both in its native, poly [→3)-α-D-GalA-(1→3)-β-L-Rha-(1→3)-α-D-Glc-O6Ac-(1→], and deacetylated forms. Initially the stretched films of both polysaccharides were amorphous, but the crystallinity was much improved by annealing at high temperature. The deacetylated specimen had higher crystallinity than the native. Both films showed similar X-ray fiber patterns indicating that these polysaccharides had similar unit cell dimensions and that the O-acetyl groups in the native beijeran chain did not disturb the regular array in the crystal having space group P21. All the visible reflections could be indexed in terms of a monoclinic unit cell with dimensions a = 1.277, b = 1.611, c (fiber axis) = 2.437 nm, and γ = 96.79 degrees. The fiber axis length and the presence of (002) and (006) reflections indicated that the conformation was made up of two trisaccharide residues, in an extended two-fold helix.

  Beijeran, Chain conformation, Fiber diffraction pattern, Unit cell parameters

  NCBI PubMed ID: 9203333
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Research Institute for Advanced Science and Technology, Osaka Prefecture University, Japan
  Methods: X-ray
  
   
  
  Azotobacter beijerinckii
  CSDB ID 7770 (all data & tools)
• Article ID: 5294
  Ogawa K, Yui T, Nakata K, Nitta Y, Kakuta M, Misaki A "Chain conformation of deacetylated beijeran calcium salt" - Bioscience, Biotechnology, and Biochemistry 60(3) (1996) 551-553
  

  A well-defined X-ray fiber diffraction pattern was obtained from a stretched film of the calcium salt of a new uronic acid containing polysaccharide designated as beijeran in the deacetylated form, poly[→3)-α-D-GalUA-(1→3)-β-L-Rham-(1→3)-α-D-Glc-(1→]. The oriented film showed no diffraction spots, indicating it to be amorphous. However, when annealed at high temperature, the film exhibited high crystallinity. All the visible reflections could be indexed in terms of a monoclinic unit cell with the following dimensions: a = 1.297; b = 1.676; c (fiber axis) = 2.509 nm; and γ = 106.50°. The length of the fiber axis and the absence of meridional reflections at any odd layer line indicate that an extended two-fold helical conformation was made up of two trisaccharide residues.

  Chain conformation, Unit cell parameters, deacetylated beijeran calcium salt, X-ray fiber pattern

  NCBI PubMed ID: 8901123
  Publication DOI: 10.1271/bbb.60.551
  Journal NLM ID: 9205717
  Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
  Institutions: Research Institute of Advanced Science and Technology, Osaka Prefecture University, Osaka, Japan, Faculty of Engineering, Osaka Prefecture University, Miyazaki, Japan, College of Agriculture, Osaka Prefecture University, Osaka, Japan, Kanan Women’s University, Kobe, Japan
  Methods: deacetylation, X-ray
  
   
  
  Azotobacter beijerinckii TNM 1
  CSDB ID 44215 (all data & tools)
• Article ID: 5791
  Knirel YA, Van Calsteren M "Bacterial exopolysaccharides" - Book: Comprehensive Glycoscience: From Chemistry to Systems Biology. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (2021) 1-75
  

  Bacterial extracellular polysaccharides are known as a cell-bound capsule, a sheath, or a slime, which is excreted into the environment. They play an important role in virulence of medical bacteria and plant-to-symbiont interaction and are used for serotyping of bacteria and production of vaccines. Some exopolysaccharides have commercial applications in industry, and claims of health benefits have been documented for an increasing number of them. Exopolysaccharides have diverse composition and structure, and some contain sugar and non-sugar components that are found in bacterial carbohydrates only. The present article provides an updated collection of the data on exopolysaccharides of various classes of gram-negative and gram-positive bacteria reported until the end of 2019. When known, biosynthesis pathways of exopolysaccharides are treated in a summary manner. References are made to structure and biosynthesis relatedness between exopolysaccharides of different bacterial taxa as well as between bacterial polysaccharides and mammalian glycosaminoglycans.

  polysaccharide structure, Gram-negative bacteria, capsule, Biofilm, polysaccharide biosynthesis, gram-positive bacteria, Monosaccharide composition, Bacterial exopolysaccharide, non-sugar component

  Publication DOI: 10.1016/B978-0-12-819475-1.00005-5
  Publisher: Elsevier
  Correspondence: marie-rose.vancalsteren@canada.ca; yknirel@gmail.com
  Editors: Barchi J, Kamerling H
  Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Hyacinthe, QC, Canada
  
   
  
  Azotobacter beijerinckii YNM1
  CSDB ID 6697 (all data & tools)