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1. (Article ID: 3637)
Ryan JM, Conrad HE
Structural heterogeneity in the lipopolysaccharide of Salmonella newington
Archives of Biochemistry and Biophysics 162(2) (1974)
530-535
Salmonella newington lipopolysaccharide extracted from a cell paste grown up from a single smooth clone was fractionated by chromatography on DEAE-cellulose in the presence of 1% Triton X-100 into seven lipopolysaccharide fractions which differed in their degrees of polymerization of the repeating unit of the O-antigen side chain and in their substitution with ester phosphate. Several of the lipopolysaccharide fractions were hydrolyzed in 1% acetic acid at 100 °C to cleave the linkage between the polysaccharide and lipid A parts of the structure. The polysaccharide fractions from each of the purified lipopolysaccharides could be further fractionated on DEAE-cellulose columns to yield a number of peaks of polysaccharide having monosaccharide ratios quite distinct from those of the parent lipopolysaccharide. The results show a high degree of structural heterogeneity in the original lipopolysaccharide.
Lipopolysaccharide, carbohydrates, lipopolysaccharides, structural, analysis, Salmonella, glucose, rhamnose, galactose, chromatography, Mannose, PDF, Glucosamine, heterogeneity, gel, Phosphates, DEAE-Cellulose, Salmonella newington, Surface-Active Agents
NCBI PubMed ID: 4407306Journal NLM ID: 0372430Institutions: Department of Biochemistry, University of Illinois, Urbana, IL, USA
Methods: sugar analysis
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