Found 1 publication.
Displayed publication 1
Expand all publications
Collapse all publications
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
1. (Article ID: 3702)
Jimenez N, Vilches S, Lacasta A, Regue M, Merino S, Tomas JM
A bifunctional enzyme in a single gene catalyzes the incorporation of GlcN into the Aeromonas core LPS
Journal of Biological Chemistry 284(48) (2009)
32995-33005
The core lipopolysaccharide (LPS) of Aeromonas hydrophila AH-3 and A. salmonicida A450 is characterized by the presence of the pentasaccharide α-D-GlcN-(1-7)-L-α-D-Hep-(1-2)-L-α-D-Hep-(1-3)-L-α-D-Hep-(1-5)-α-Kdo. Previously it has been suggested that the WahA protein is involved in the incorporation of GlcN residue to outer core lipopolysaccharide (LPS). The WahA protein contains two domains: a glycosyltransferase and a carbohydrate esterase. In this work we demonstrate that the independent expression of the WahA glycosyltransferase domain catalyzes the incorporation of GlcNAc from UDP-GlcNAc to the outer core LPS. Independent expression of the carbohydrate esterase domain leads to the deacetylation of the GlcNAc residue to GlcN. Thus, the WahA is the first described bifunctional glycosyltransfease enzyme involved in the biosynthesis of core LPS. By contrast in Enterobacteriaceae containing GlcN in their outer core LPS the two reactions are performed by two different enzymes
Lipopolysaccharide, biosynthesis, core, glycosyltransferase, Aeromonas hydrophila
NCBI PubMed ID: 19805547Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: jtomas
ub.edu
Institutions: Department Microbiologia University Barcelona, Spain
Methods: SDS-PAGE, MALDI-TOF MS, genetic methods, biochemical methods, mild acid degradation
The publication contains the following compound(s):
- Compound ID: 8484
|
a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+
|
D-gro-a-D-manHepp-(1-6)-+ | P-4)-+
| | |
Subst-(1-?)-b-D-Galp-(1-4)-D-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/
|
L-gro-a-D-manHepp-(1-6)-+
Subst = O-antigen |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Compound class: core oligosaccharide, LPS
- Compound ID: 8485
|
L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+
|
?%b-D-Galp-(1-4)-+ |
| |
?%D-gro-a-D-manHepp-(1-6)-?%D-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdo
|
L-gro-a-D-manHepp-(1-6)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Reference(s) to other database(s): GTC:G37238EU, GlycomeDB:
37354
- Compound ID: 8486
|
a-D-GlcpNAc-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+
|
b-D-Galp-(1-4)-+ |
| |
D-gro-a-D-manHepp-(1-6)-D-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdo
|
L-gro-a-D-manHepp-(1-6)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Reference(s) to other database(s): GTC:G01756XE, GlycomeDB:
37355
- Compound ID: 8487
|
a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-2)-L-gro-a-D-manHepp-(1-3)-+
|
a-D-Galp-(1-6)-+ | P-4)-+
| | |
Subst-(1-?)-b-D-GalpNAc-(1-4)-D-gro-a-D-manHepp-(1-6)-b-D-Glcp-(1-4)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/
|
L-gro-a-D-manHepp-(1-6)-+
Subst = O-antigen |
Show graphically |
Structure type: oligomer
Aglycon: lipid A
Compound class: LPS
Expand this publication
Collapse this publication
Total list of publication IDs on all result pages of the current query:
Total list of corresponding CSDB IDs (permanent record IDs):
Execution: 2 sec