The electrophoretic behavior in polyacrylamide gels of the acidic polysaccharides produced by the soil bacteria Bradyrhizobium (Chamaecytisus) strain BGA1 and Bradyrhizobium japonicum USDA1 10 has been studied. Both polysaccharides were polydisperse, producing a ladder-like pattern after fixation with Alcian Blue and silver staining of the gel. The polysaccharide molecules were separated according to their size, and they behaved as a collection of flexible random coils of different size and similar charge/mass ratio. The electrophoretic behavior was not affected by the presence of acetyl groups in the polysaccharide. The range of molecular weights of the exopolysaccharide produced by B. japonicum USDA110 was wider and with larger molecules than that of the polysaccharide produced by strain BGA1. The resolution was dependent on the electrophoresis buffer; the best results were achieved with Tris-borate; in Tris-glycine buffer, the resolution was worse, and it was not improved by the addition of sodium dodecyl sulfate (SDS).
strain, polysaccharide, polysaccharides, acidic, acidic polysaccharide, bacteria, exopolysaccharides, soil bacteria, size, distribution, behavior, Bradyrhizobium japonicum, Bradyrhizobium, acidic polysaccharides, electrophoretic, polyacrylamide gel electrophoresis, size distribution
NCBI PubMed ID: 9848669Journal NLM ID: 8204476Publisher: Wiley-VCH
Correspondence: fcorzo@ull.es
Institutions: Departamento de Bioqufmica y Biologia Molecular, Universidad de La Laguna
Methods: PAGE
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