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1. (Article ID: 3822)
 
Boiko AS, Smol'kina ON, Fedonenko YP, Zdorovenko EL, Kachala VV, Konnova SA, Ignatov VV
O-polysaccharide structure in serogroup I Azospirilla
Mikrobiologiia = Microbiology [Russian] 79(2) (2010) 197-205
 

Lipopolysaccharides (LPS) and O-specific polysaccharides (OPS) were obtained from the outermembrane of four Azospirillum strains previously assigned to serogroup I based on the serological affinityrevealed by the antibodies (AB) to the LPS of A. brasilense Sp245. Investigation, including determination ofmonosaccharide composition, methylation analysis, and one- and two-dimensional NMR spectroscopy, wascarried out to determine the OPS structure. The OPSs of A. brasilense Sp107 and S27 and of A. lipoferumRG20a were found to have an identical structure of repeating units represented by a linear penta-D-rhamnan,as was previously described for the OPSs of A. brasilense Sp245 and SR75. The OPS of A. brasilense SR15was found to consist of tetrasaccharide repeating units of the following structure: frame0 2)--D-Rhap-(1 frame1 2)--D-Rhap-(1 frame2 3)--D-Rhap-(1 frame3 2)--D-Rhap-(1 frame4. An opine compound, N-(1-carboxyethyl)-orni-thine, closely associated with the LPS of A. brasilense SR15, was identified in azospirilla for the first time. Thepresence of a 6-deoxisugar (D-rhamnose) in the OPS structure was shown to be the chemical basis of the sero-logical similarity and the reason for classification of these strains within the serogroup I.

Lipopolysaccharide, serological characterization, Azospirillum, structure of O-specific polysaccharides

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