The Cronobacter spp., previously known as Enterobacter sakazakii, are Gram-negative enterobacterial pathogens that can cause necrotizing enterocolitis, meningitis, and septicemia with a high mortality rate in neonates. The O-specific polysaccharide (O-PS) was isolated from Cronobacter sakazakii strain 767 and structurally characterized using 1H and 13C NMR spectroscopy, including two-dimensional DQF-COSY, TOCSY, ROESY, HSQC, andHMBC experiments. Further compositional determination was undertaken using classical chemical methods followed by GLC, and GLC-MS analysis. The repeating unit of O-PS isolated from C. sakazakii 767 was a branched heptasaccharide composed of L-Rha, D-Glc, D-GlcNAc, and D-GalA, and had the structure shown below. One of the Rha residues was partially O-acetylated at C-4. C. sakazakii 767 was originally isolated from a fatal neonatal meningitic case, and the structure of its O-PS significantly differs from the O-PS structures previously described for Cronobacter spp.

Lipopolysaccharide, O-antigen, NMR spectroscopy, bacterial polysaccharide structure, Cronobacter sakazakii, Enterobacter sakazakii

• Compound ID: 8892
  

  a-L-Rhap-(1-4)-a-D-GalpA-(1-2)-+ | a-D-Glcp-(1-4)-+ | | | -3)-b-D-GlcpNAc-(1-3)-a-L-Rhap4(50%)Ac-(1-4)-a-D-Glcp-(1-2)-a-L-Rhap-(1-

  Show graphically

  Show legend Show as text

  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide
  Reference(s) to other database(s): GTC:G20299ZC
  
   
  
  Cronobacter sakazakii 767
  CSDB ID 25223 (all data & tools)