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Gudlavalleti SK, Szymanski CM, Jarrell HC, Stephens DS
In vivo determination of Neisseria meningitidis serogroup A capsular polysaccharide by whole cell high-resolution magic angle spinning NMR spectroscopy
Carbohydrate Research 341(4) (2006)
557-562
Neisseria meningitidis A (F8229)
(Ancestor NCBI TaxID 65699,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Neisseria meningitidis [ICD11:
XN1DV 
]
NCBI PubMed ID: 16406275Publication DOI: 10.1016/j.carres.2005.11.036Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: David S. Stephens <dstep01
emory.edu>
Institutions: Laboratories of Microbial Pathogenesis, VA Medical Center and Division of Infectious Diseases, Atlanta, GA, USA, Departments of Medicine, Emory University School of Medicine, Atlanta, GA, USA
High resolution-magic angle spinning (HRMAS) NMR spectroscopy was applied to serogroup A Neisseria meningitidis (NMA) to determine precise structures of capsular polysaccharide (CPS) expressed on the meningococcal surface. Both the O-acetylated (OAc) NMA parent and a mynC::aphA3 OAc- mutant demonstrated characteristic CPS-derived NMR signals indicating cell-surface expression of CPS, but only the parent expressed O-3 and O-4 acetylation signals. A capsule-defective strain showed no NMR signals for CPS. The (1)H NMR HRMAS spectral patterns correlated with the purified CPS (1)H NMR profiles. HRMAS NMR can distinguish detailed complex carbohydrate structures expressed on bacteria. NMA express both O-3 and O-4 acetylated polymers but not in equimolar ratio amounts in vivo.
Neisseria meningitidis, meningococci, capsular polysaccharide, O-acetylation, HRMAS NMR spectroscopy, vaccine development
Structure type: homopolymer
Location inside paper: graphical abstract
Methods: HR-MAS NMR, 1H HR-MAS NMR, cell surface hydrophobicity
Related record ID(s): 20165
NCBI Taxonomy refs (TaxIDs): 65699Reference(s) to other database(s): GTC:G99718IV
Show glycosyltransferases
NMR conditions: in D2O at 294(H) K
[as TSV]
1H NMR data: present in publication
|
There is only one chemically distinct structure:
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Gudlavalleti SK, Szymanski CM, Jarrell HC, Stephens DS
In vivo determination of Neisseria meningitidis serogroup A capsular polysaccharide by whole cell high-resolution magic angle spinning NMR spectroscopy
Carbohydrate Research 341(4) (2006)
557-562
Neisseria meningitidis A
(NCBI TaxID 65699,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Neisseria meningitidis [ICD11:
XN1DV ]
NCBI PubMed ID: 16406275Publication DOI: 10.1016/j.carres.2005.11.036Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: David S. Stephens <dstep01
emory.edu>
Institutions: Laboratories of Microbial Pathogenesis, VA Medical Center and Division of Infectious Diseases, Atlanta, GA, USA, Departments of Medicine, Emory University School of Medicine, Atlanta, GA, USA
High resolution-magic angle spinning (HRMAS) NMR spectroscopy was applied to serogroup A Neisseria meningitidis (NMA) to determine precise structures of capsular polysaccharide (CPS) expressed on the meningococcal surface. Both the O-acetylated (OAc) NMA parent and a mynC::aphA3 OAc- mutant demonstrated characteristic CPS-derived NMR signals indicating cell-surface expression of CPS, but only the parent expressed O-3 and O-4 acetylation signals. A capsule-defective strain showed no NMR signals for CPS. The (1)H NMR HRMAS spectral patterns correlated with the purified CPS (1)H NMR profiles. HRMAS NMR can distinguish detailed complex carbohydrate structures expressed on bacteria. NMA express both O-3 and O-4 acetylated polymers but not in equimolar ratio amounts in vivo.
Neisseria meningitidis, meningococci, capsular polysaccharide, O-acetylation, HRMAS NMR spectroscopy, vaccine development
Structure type: homopolymer
Location inside paper: graphical abstract
Methods: HR-MAS NMR, 1H HR-MAS NMR, cell surface hydrophobicity
Related record ID(s): 387
NCBI Taxonomy refs (TaxIDs): 65699Reference(s) to other database(s): GTC:G98670IQ
Show glycosyltransferases
NMR conditions: in D2O at 294(H) K
[as TSV]
1H NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
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