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1. (Article ID: 3960)
 
Vinogradov E, King JD, Pathak AK, Harvill ET, Preston A
Antigenic Variation among Bordetella: Bordetella bronchiseptica strain MO149 expresses a novel O chain that is poorly immunogenic
Journal of Biological Chemistry 285(35) (2010) 26869-26877
 

The O chain polysaccharide (O PS) of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharide is a homopolymer of 2,3-diacetamido-2,3-dideoxygalacturonic acid (GalNAc3NAcA) in which some of the sugars are present as uronamides. The terminal residue contains several unusual modifications. To date, two types of modification have been characterized, and a survey of numerous strains demonstrated that each contained one of these two modification types. Host antibody responses against the O PS are directed against the terminal residue modifications, and there is little cross-reactivity between the two types. This suggests that Bordetella O PS modifications represent a means of antigenic variation. Here we report the characterization of the O PS of B. bronchiseptica strain MO149. It consists of a novel two-sugar repeating unit and a novel terminal residue modification, with the structure Me-4-α-L-GalNAc3NAcA-(4-β-D-GlcNAc3NAcA-4-α-L-GalNAc3NAcA-)(5-6)-, which we propose be defined as the B. bronchiseptica O3 PS. We show that the O3 PS is very poorly immunogenic and that the MO149 strain contains a novel wbm (O PS biosynthesis) locus. Thus, there is greater diversity among Bordetella O PSs than previously recognized, which is likely to be a result of selection pressure from host immunity. We also determine experimentally, for the first time, the absolute configuration of the diacetimido-uronic acid sugars in Bordetella O PS.

O polysaccharide, bacteria, Bordetella, immunity, lipopolysaccharide (LPS), evolution, Bacterial Genetics, Humoral Response

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