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Zdorovenko EL, Kadykova AA, Shashkov AS, Varbanets LD, Bulyhina TV, Knirel YA
Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity
Carbohydrate Research 496 (2020)
108132
Pantoea agglomerans 7460
(Ancestor NCBI TaxID 549,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 32861900Publication DOI: 10.1016/j.carres.2020.108132Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E.L. Zdorovenko <zdorovenkoe
mail.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences, 154 Zabolotnoho Str., 03143, Kiev, Ukraine
Lipopolysaccharide (LPS) was isolated from Pantoea agglomerans 7460 cells by phenol-water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated derivative containing biphosphorylated GlcN disaccharide, four 14:0 (3-OH), 18:0 and 12:0 fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR and computational methods: The LPS of capital ER, Cyrillic. agglomerans 7460 showed low level of toxicity and pyrogenicity to compare with LPS of E. coli O55:B5 and pyrogenal, respectively. The ability of the modified (succinylated) LPS, which have lost its toxicity, to block the toxic effects of native LPS has been shown.
lipid A, Pantoea agglomerans, toxicity, O-Polysaccharide structure, Pyrogenicity, Anti-LPS strategy
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_1394181,IEDB_141794,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, ESI-MS, composition analysis, serological methods, GPC, assay of polymyxin sensitivity, pyrogenicity assays, succinylation
Comments, role: minor OPS
Related record ID(s): 32242
NCBI Taxonomy refs (TaxIDs): 549Reference(s) to other database(s): GTC:G57042EP
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,2 aDRhap 101.5 79.1 70.7 73.6 70.6 17.9
4 aDRhap 102.0 79.8 71.3 73.6 70.6 17.8
bDGalp 103.8 71.7 73.1 78.8 76.7 62.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,2 aDRhap 5.11 4.261 3.83 3.47 3.72 1.30
4 aDRhap 5.17 4.12 3.89 3.48 3.71 1.31
bDGalp 4.51 3.56 3.74 4.02 3.77 3.76-3.77
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,2 aDRhap 101.5/5.11 79.1/4.261 70.7/3.83 73.6/3.47 70.6/3.72 17.9/1.30
4 aDRhap 102.0/5.17 79.8/4.12 71.3/3.89 73.6/3.48 70.6/3.71 17.8/1.31
bDGalp 103.8/4.51 71.7/3.56 73.1/3.74 78.8/4.02 76.7/3.77 62.0/3.76-3.77
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,2 | aDRhap | 5.11 | 4.261 | 3.83 | 3.47 | 3.72 | 1.30 |
| 4 | aDRhap | 5.17 | 4.12 | 3.89 | 3.48 | 3.71 | 1.31 |
| | bDGalp | 4.51 | 3.56 | 3.74 | 4.02 | 3.77 | 3.76
3.77 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,2 | aDRhap | 101.5 | 79.1 | 70.7 | 73.6 | 70.6 | 17.9 |
| 4 | aDRhap | 102.0 | 79.8 | 71.3 | 73.6 | 70.6 | 17.8 |
| | bDGalp | 103.8 | 71.7 | 73.1 | 78.8 | 76.7 | 62.0 |
|
There is only one chemically distinct structure:
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Palčeková Z, Obregón-Henao A, De K, Walz A, Lam H, Philp J, Angala SR, Patterson J, Pearce C, Zuberogoitia S, Avanzi C, Nigou J, McNeil M, Muñoz Gutiérrez JF, Gilleron M, Wheat WH, Gonzalez-Juarrero M, Jackson M
Role of succinyl substituents in the mannose-capping of lipoarabinomannan and control of inflammation in Mycobacterium tuberculosis infection
PLoS Pathogens 19(9) (2023)
e1011636
|
{{{-a-D-Manp-(1-2)-}}}/n=6-7/-a-D-Manp-(1-2)-+
|
b-D-Araf-(1-2)-a-D-Araf-(1-3)-+ |
| |
a-D-Manp-(1-2)-a-D-Manp-(1-2)-b-D-Araf-(1-2)-a-D-Araf-(1-5)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-3)-+ |
| |
D-Manp-(1-?)-b-D-Araf-(1-2)-a-D-Araf-(1-5)-+ | |
| | |
Suc-(1-3)-+ | | |
| | | |
a-D-Xylf5S5Me-(1-4)-a-D-Manp-(1-2)-a-D-Manp-(1-5)-b-D-Araf-(1-2)-a-D-Araf-(1-3)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-5)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-5)-+ |
| | |
Suc-(1-2)-+ | |
| |
b-D-Araf-(1-2)-a-D-Araf-(1-3)-+ | |
| | |
b-D-Araf-(1-2)-{{{-a-D-Araf-(1-5)-}}}/n=5/-a-D-Araf-(1-5)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-3)-+ | |
| | |
Suc-(1-3)-+ | | |
| | | |
Suc-(1-3)-+ a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Araf-(1-2)-b-D-Araf-(1-5)-a-D-Araf-(1-5)-+ | | a-D-Manp-(1-2)-+ | a-D-Manp-(1-2)-+ a-D-Manp-(1-2)-+
| | | | | | | |
b-D-Araf-(1-2)-a-D-Araf-(1-5)-a-D-Araf-(1-5)-a-D-Araf-(1-5)-{{{-D-Araf-(1-?)-}}}a-D-Araf-(1-3)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-5)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-3)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}/n=13/-a-D-Araf-(1-6)-a-D-Manp-(1-6)-{{{-a-D-Manp-(1-6)-}}}/n=2/-a-D-Manp-(1-6)-{{{-a-D-Manp-(1-6)-}}}a-D-Manp-(1-6)-{{{-a-D-Manp-(1-6)-}}}/n=2/-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1--/phosphatidyl-myo-inositol anchor/
|
Suc-(1-2)-+
Xyl5S = 5-thio-xylose |
Show graphically |
Mycobacterium tuberculosis
(NCBI TaxID 1773,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Associated disease: infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 
]
NCBI PubMed ID: 37669276Publication DOI: 10.1371/journal.ppat.1011636Journal NLM ID: 101238921Publisher: San Francisco, CA: Public Library of Science
Correspondence: M. Jackson <Mary.Jackson
colostate.edu>
Institutions: Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America, Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
The covalent modification of bacterial (lipo)polysaccharides with discrete substituents may impact their biosynthesis, export and/or biological activity. Whether mycobacteria use a similar strategy to control the biogenesis of its cell envelope polysaccharides and modulate their interaction with the host during infection is unknown despite the report of a number of tailoring substituents modifying the structure of these glycans. Here, we show that discrete succinyl substituents strategically positioned on Mycobacterium tuberculosis (Mtb) lipoarabinomannan govern the mannose-capping of this lipoglycan and, thus, much of the biological activity of the entire molecule. We further show that the absence of succinyl substituents on the two main cell envelope glycans of Mtb, arabinogalactan and lipoarabinomannan, leads to a significant increase of pro-inflammatory cytokines and chemokines in infected murine and human macrophages. Collectively, our results validate polysaccharide succinylation as a critical mechanism by which Mtb controls inflammation.
lipoarabinomannan, Mycobacterium tuberculosis, tuberculosis
Structure type: structural motif or average structure
Location inside paper: Fig. 1, lipoarabinomannan (LAM)
Aglycon: phosphatidyl-myo-inositol anchor
Trivial name: lipoarabinomannan (LAM)
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_1309625,IEDB_1309626,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141795,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_141834,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_164480,IEDB_76933,IEDB_857718,IEDB_857732,IEDB_857735,IEDB_857736,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_72
Methods: GC-MS, SDS-PAGE, composition analysis, genetic methods, biochemical methods, enzyme assay, statistical analysis, LC-MS, flow cytometry analysis, determination of NO production, macrophage activity assay, genome sequencing, succinylation, UPLC
Related record ID(s): 25991
NCBI Taxonomy refs (TaxIDs): 1773
Show glycosyltransferases
SMILES errors: xXSuc(1-2)[bDAraf(1-2)aDAraf(1-3)[aDManp(1-2)aDManp(1-2)bDAraf(1-2)aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-3)[xXSuc(1-2)[?DManp(1-?)bDAraf(1-2)aDAraf(1-5),Me(1-5)aDXylf5S(1-4)aDManp(1-2)aDManp(1-5)bDAraf(1-2)[xXSuc(1-3)]aDAraf(1-3)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-5),bDAraf(1-2)aDAraf(1-3)[bDAraf(1-2)/aDAraf(1-5)/n=5/aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-3)[bDAraf(1-2)[xXSuc(1-3)]aDAraf(1-5)aDAraf(1-5)aDAraf(1-5)/?DAraf(1-?)/n=?/aDAraf(1-3)[aDManp(1-2)aDManp(1-2)aDAraf(1-2)[xXSuc(1-3)]bDAraf(1-5)aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-3)]aDAraf(1-5)/aDAraf(1-5)/n=13/aDAraf(1-6)aDManp(1-6)/[aDManp(1-2)]aDManp(1-6)/n=2/[/aDManp(1-2)/n=6-7/aDManp(1-2)]aDManp(1-6)/[aDManp(1-2)]aDManp(1-6)/n=?/aDManp(1-6)/[aDManp(1-2)]aDManp(1-6)/n=2/aDManp(1-6)aDManp(1-6)aDManp // Xyl5S = 5-thio-xylose:
SMILES error: number of atoms (~992) exceeds a structural formula limit (500)
Expand this record
Collapse this record
Palčeková Z, Obregón-Henao A, De K, Walz A, Lam H, Philp J, Angala SR, Patterson J, Pearce C, Zuberogoitia S, Avanzi C, Nigou J, McNeil M, Muñoz Gutiérrez JF, Gilleron M, Wheat WH, Gonzalez-Juarrero M, Jackson M
Role of succinyl substituents in the mannose-capping of lipoarabinomannan and control of inflammation in Mycobacterium tuberculosis infection
PLoS Pathogens 19(9) (2023)
e1011636
|
b-D-Araf-(1-2)-a-D-Araf-(1-5)-+
|
b-D-Araf-(1-2)-a-D-Araf-(1-3)-a-D-Araf-(1-5)-a-D-Araf-(1-5)-a-D-Araf-(1-5)-+
| |
?%Suc-(1-2)-+ |
|
b-D-Araf-(1-2)-a-D-Araf-(1-5)-+ |
| |
b-D-Araf-(1-2)-a-D-Araf-(1-3)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-3)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}/n=12/-a-D-Araf-(1-5)-+
| |
?%Suc-(1-2)-+ |
|
Mycolic-(1-?)-b-D-Araf-(1-2)-a-D-Araf-(1-5)-+ |
| |
Mycolic-(1-?)-b-D-Araf-(1-2)-a-D-Araf-(1-3)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-5)-+ |
| |
Mycolic-(1-?)-b-D-Araf-(1-2)-a-D-Araf-(1-5)-+ | |
| | |
Mycolic-(1-?)-b-D-Araf-(1-2)-a-D-Araf-(1-3)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}a-D-Araf-(1-3)-a-D-Araf-(1-5)-a-D-Araf-(1-5)-{{{-a-D-Araf-(1-5)-}}}/n=12/-a-D-Araf-(1-5)-+ |
| | |
?%a-D-GalpN-(1-2)-+ | |
| |
Subst1-(1-?)-Subst2-(1-?)-b-D-Galf-(1-5)-{{{-b-D-Galf-(1-6)-b-D-Galf-(1-5)-}}}/n=2/-b-D-Galf-(1-6)-b-D-Galf-(1-5)-b-D-Galf-(1-6)-b-D-Galf-(1-5)-{{{-b-D-Galf-(1-6)-b-D-Galf-(1-5)-}}}/n=5/-b-D-Galf-(1-6)-b-D-Galf
Subst1 = peptidoglycan;
Subst2 = linker |
Show graphically |
Mycobacterium tuberculosis
(NCBI TaxID 1773,
species name lookup)
Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Associated disease: infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 ]
NCBI PubMed ID: 37669276Publication DOI: 10.1371/journal.ppat.1011636Journal NLM ID: 101238921Publisher: San Francisco, CA: Public Library of Science
Correspondence: M. Jackson <Mary.Jackson
colostate.edu>
Institutions: Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America, Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
The covalent modification of bacterial (lipo)polysaccharides with discrete substituents may impact their biosynthesis, export and/or biological activity. Whether mycobacteria use a similar strategy to control the biogenesis of its cell envelope polysaccharides and modulate their interaction with the host during infection is unknown despite the report of a number of tailoring substituents modifying the structure of these glycans. Here, we show that discrete succinyl substituents strategically positioned on Mycobacterium tuberculosis (Mtb) lipoarabinomannan govern the mannose-capping of this lipoglycan and, thus, much of the biological activity of the entire molecule. We further show that the absence of succinyl substituents on the two main cell envelope glycans of Mtb, arabinogalactan and lipoarabinomannan, leads to a significant increase of pro-inflammatory cytokines and chemokines in infected murine and human macrophages. Collectively, our results validate polysaccharide succinylation as a critical mechanism by which Mtb controls inflammation.
lipoarabinomannan, Mycobacterium tuberculosis, tuberculosis
Structure type: structural motif or average structure
Location inside paper: Fig. 1, arabinogalactan (AG)
Trivial name: arabinogalactan (AG)
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_1309625,IEDB_134619,IEDB_136095,IEDB_137472,IEDB_137473,IEDB_149176,IEDB_159255,IEDB_190606,IEDB_857717,IEDB_857718,IEDB_857720,IEDB_885812
Methods: GC-MS, SDS-PAGE, composition analysis, genetic methods, biochemical methods, enzyme assay, statistical analysis, LC-MS, flow cytometry analysis, determination of NO production, macrophage activity assay, genome sequencing, succinylation, UPLC
Related record ID(s): 22287
NCBI Taxonomy refs (TaxIDs): 1773
Show glycosyltransferases
SMILES errors: %xXSuc(1-2)[%xXSuc(1-2)[bDAraf(1-2)aDAraf(1-5),bDAraf(1-2)aDAraf(1-3)]aDAraf(1-5)aDAraf(1-5)aDAraf(1-5),bDAraf(1-2)aDAraf(1-3)[bDAraf(1-2)aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-3)]aDAraf(1-5)/aDAraf(1-5)/n=12/aDAraf(1-5)[%aDGalpN(1-2)[lXMycolic(1-?)bDAraf(1-2)aDAraf(1-3)[lXMycolic(1-?)bDAraf(1-2)aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-5),lXMycolic(1-?)bDAraf(1-2)aDAraf(1-3)[lXMycolic(1-?)bDAraf(1-2)aDAraf(1-5)]aDAraf(1-5)/aDAraf(1-5)/n=?/aDAraf(1-3)]aDAraf(1-5)aDAraf(1-5)/aDAraf(1-5)/n=12/aDAraf(1-5)[Subst1(1-?)Subst2(1-?)bDGalf(1-5)/bDGalf(1-6)bDGalf(1-5)/n=2/bDGalf(1-6)]bDGalf(1-5)bDGalf(1-6)]bDGalf(1-5)/bDGalf(1-6)bDGalf(1-5)/n=5/bDGalf(1-6)bDGalf // Subst1 = peptidoglycan; Subst2 = linker:
SMILES error: number of atoms (~852) exceeds a structural formula limit (500)
Expand this record
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Zdorovenko EL, Kadykova AA, Shashkov AS, Varbanets LD, Bulyhina TV, Knirel YA
Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity
Carbohydrate Research 496 (2020)
108132
|
70%a-D-Galp-(1-3)-+
|
-2)-a-D-Rhap-(1-2)-a-D-Rhap-(1-4)-b-D-Galp-(1- |
Show graphically |
Pantoea agglomerans 7460
(Ancestor NCBI TaxID 549,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 32861900Publication DOI: 10.1016/j.carres.2020.108132Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E.L. Zdorovenko <zdorovenkoe
mail.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences, 154 Zabolotnoho Str., 03143, Kiev, Ukraine
Lipopolysaccharide (LPS) was isolated from Pantoea agglomerans 7460 cells by phenol-water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated derivative containing biphosphorylated GlcN disaccharide, four 14:0 (3-OH), 18:0 and 12:0 fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR and computational methods: The LPS of capital ER, Cyrillic. agglomerans 7460 showed low level of toxicity and pyrogenicity to compare with LPS of E. coli O55:B5 and pyrogenal, respectively. The ability of the modified (succinylated) LPS, which have lost its toxicity, to block the toxic effects of native LPS has been shown.
lipid A, Pantoea agglomerans, toxicity, O-Polysaccharide structure, Pyrogenicity, Anti-LPS strategy
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_136044,IEDB_136906,IEDB_137472,IEDB_1394181,IEDB_141794,IEDB_151528,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, ESI-MS, composition analysis, serological methods, GPC, assay of polymyxin sensitivity, pyrogenicity assays, succinylation
Comments, role: major OPS
Related record ID(s): 3974
NCBI Taxonomy refs (TaxIDs): 549Reference(s) to other database(s): GTC:G16751QX
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,2 aDRhap 100.0 79.5 70.8 73.6 70.8 18.0
4,3 70%aDGalp 102.1 70.5 69.7 70.7 73.2 62.8
4 aDRhap 101.5 76.2 78.9 73.3 70.7 17.7
bDGalp 104.3 71.7 73.0 78.7 76.7 62.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,2 aDRhap 5.29 4.21 3.83 3.49 3.71 1.34
4,3 70%aDGalp 5.33 3.87 3.82 4.01 3.97 3.74-3.80
4 aDRhap 4.85 4.27 4.03 3.76 4.18 1.28
bDGalp 4.49 3.60 3.79 4.02 3.77 3.76-3.77
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,2 aDRhap 100.0/5.29 79.5/4.21 70.8/3.83 73.6/3.49 70.8/3.71 18.0/1.34
4,3 70%aDGalp 102.1/5.33 70.5/3.87 69.7/3.82 70.7/4.01 73.2/3.97 62.8/3.74-3.80
4 aDRhap 101.5/4.85 76.2/4.27 78.9/4.03 73.3/3.76 70.7/4.18 17.7/1.28
bDGalp 104.3/4.49 71.7/3.60 73.0/3.79 78.7/4.02 76.7/3.77 62.0/3.76-3.77
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,2 | aDRhap | 5.29 | 4.21 | 3.83 | 3.49 | 3.71 | 1.34 |
| 4,3 | 70%aDGalp | 5.33 | 3.87 | 3.82 | 4.01 | 3.97 | 3.74
3.80 |
| 4 | aDRhap | 4.85 | 4.27 | 4.03 | 3.76 | 4.18 | 1.28 |
| | bDGalp | 4.49 | 3.60 | 3.79 | 4.02 | 3.77 | 3.76
3.77 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,2 | aDRhap | 100.0 | 79.5 | 70.8 | 73.6 | 70.8 | 18.0 |
| 4,3 | 70%aDGalp | 102.1 | 70.5 | 69.7 | 70.7 | 73.2 | 62.8 |
| 4 | aDRhap | 101.5 | 76.2 | 78.9 | 73.3 | 70.7 | 17.7 |
| | bDGalp | 104.3 | 71.7 | 73.0 | 78.7 | 76.7 | 62.0 |
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Total list of record IDs on all result pages of the current query:
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