From the lipopolysaccharides (LPSs) of the plant-pathogenic bacterium Burkholderia caryophylli, the complete structure of lipid A has been characterized. For the first time, a 4-amino-4-deoxy-L-arabinopyranose 1-phosphate residue was proven to be exclusively linked to the reducing end of lipid A from a wild-type LPS. The LPSs of B. caryophylli were degraded by mild acetate buffer hydrolysis at pH 4.4. The obtained lipid A was analyzed as such, and also after de-O-acylation or dephosphorylation. The structure of lipid A was identified mainly by means of matrix-assisted laser desorption/ionisation mass spectrometry, and by various 1D and 2D (1)H and (13)C NMR spectroscopic measurements.
lipopolysaccharides, lipid A, NMR spectroscopy, mass spectrometry, Burkholderia caryophilli
NCBI PubMed ID: 12658652Publication DOI: 10.1002/chem.200390177Journal NLM ID: 9513783Publisher: Weinheim: VCH Verlagsgesellschaft/Verlag I
Correspondence: oholst@fz-borstel.de
Institutions: Divison of Structural Biochemistry, Research Center Borstel, Center for Medicine and Biosciences Parkallee 22, 23845 Borstel (Germany), Dipartimento di Chimica Organica e Biochimica, Università degli studi di Napoli Federico II, Italy
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, HF solvolysis, TLC, 31P NMR, GLC, chemical methods, MALDI-TOF MS, de-O-acetylation, NMR-1D
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