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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Associated disease: apple silver-leaf disease

Publication DOI: 10.1271/bbb.62.852
Journal NLM ID: 9205717
Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Institutions: Department of Bioresources Science, Faculty of Agriculture, Hirosaki University, Hirosaki, Aomori 036-8561, Japan

Three endopolygalacturonases (endoPG Ia, Ib, and Ic) were isolated from the culture filtrate of Stereum purpureum, the causative fungus of apple silver-leaf disease. Their properties, including specific activities, optimum pHs, thermal stabilities, and kinetic parameters (K(m) and Vmax) were compared. Their properties were very similar to one another except for the substrate specificity and relative molecular mass. The sugar chains of endoPG Is were released by hydrazinolysis, and one major sugar chain common to endoPG Is was isolated. The pyridylamino sugar was characterized by a two-dimensional mapping method using HPLC, and identified as a high mannose type N-linked sugar chain, Man alpha 1-6(Man alpha 1-3)Man alpha 1-6(Man alpha 1-3) Man beta 1-4 GlcNAc beta 1-4 GlcNAc (designated as M5.1). Observation of the course of Western blot analysis for the proteins from the culture filtrate with endoPG I antibodies showed that the fungus secreted three endoPG Is into the culture broth during the growing period.

endopolygalacturonase Ia, endopolygalacturonase Ib, endopolygalacturonase Ic, N-linked sugar chain, Stereum purpureum

Structure type: oligomer ; 1313.8 [M*H]+
Location inside paper: Fig. 6, M5.1
Contained glycoepitopes: IEDB_123886,IEDB_130701,IEDB_135813,IEDB_137340,IEDB_137485,IEDB_1394182,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_144983,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153212,IEDB_153220,IEDB_164174,IEDB_187201,IEDB_429156,IEDB_548907,IEDB_857734,IEDB_983930,SB_197,SB_198,SB_33,SB_44,SB_67,SB_72,SB_73,SB_74,SB_77,SB_85

Methods: gel filtration, SDS-PAGE, acid hydrolysis, HPLC, hydrazinolysis, column chromatography, enzymatic activity assay, western blot, isoelectric focusing, ion spray MS
Comments, role: sugar chain of endoPG Ia, Ib, and Ic

NCBI Taxonomy refs (TaxIDs): 58369
Reference(s) to other database(s): GTC:G66305IS
Show glycosyltransferases

There are too many chemically distinct structures (~24576), so only one is shown:

aDManp(1-3)[aDManp(1-3)[aDManp(1-6)]aDManp(1-6)]bDManp(1-4)[Ac(1-2)]bDGlcpN(1-4)[Ac(1-2)]?DGlcpN

CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO[C@H]4O[C@H](CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@H](O)[C@H]2NC(C)=O)[C@@H]1O

SVG MW SMILES 3D mol Ionize