Schweiger-Hufnagel U, Ono T, Izumi K, Hufnagel P, Morita N, Kaga H, Morita M, Hoshino T, Yumoto I, Matsumoto N, Yoshida M, Sawada MT, Okuyama H Identification of the extracellular polysaccharide produced by the snow mold fungus Microdochium nivale Biotechnology Letters22 (2000)
183-187
Publication DOI:10.1023/A:1005647928900 Journal NLM ID:8008051 Publisher: Kluwer Academic Publishers Correspondence: hokuees.hokudai.ac.jp Institutions: Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, AIST/MITI, Toyohira-ku, Sapporo, 062-8517, Japan, Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan, National Institute of Agro-Environmental Sciences, Kan-non dai, Tsukuba, 305-8604, Japan, Hokkaido National Agricultural Experiment Station, Toyohira-ku, Sapporo, 062-8555, Japan
A water-insoluble, extracellular polysaccharide was isolated from the culture medium of the snow mold fungus, Microdochium nivale, that had been cultivated in potato/dextrose broth. The polysaccharide consisted of glucose only. Its Fourier transform infrared spectrum showed a beta configuration of the C1 position of glucose. Linkage analysis of the polysaccharide showed that it had a linear structure of β-(1→4)-linked glucose. The polysaccharide was therefore identified as cellulose. This is the first report of extracellular cellulose occurring in fungi.
Bao XF, Duan JY, Fang XY, Fang JN Chemical modifications of the (1→3)-α-d-glucan from spores of Ganoderma lucidum and investigation of their physicochemical properties and immunological activity Carbohydrate Research336 (2001)
127-140
Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota) Organ / tissue:spore
The structure was elucidated in this paper Journal NLM ID:0043535 WWW link:http://www.sciencedirect.com/science/article/pii/S0008621501002385 Publisher: Elsevier Correspondence: jnfangmail.shcnc.ac.cn Institutions: Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 294 Tai-Yuan Road, 200031, P.R., Shanghai, China
A linear (1→3)-α-d-glucan was isolated from the spores of Ganoderma lucidum (Fr.) Karst. Six different functionalized derivatives of the (1→3)-α-d-glucan—aminopropylated, hydroxyethylated, sulfated, carboxymethylated, carboxymethylated and sulfated, and benzylamidated–carboxymethylated—with varying degrees of substitution were synthesized. The structural features and physicochemical properties of all derivatives were investigated by means of chemical and spectral analyses, and their effects on lymphocyte proliferation and antibody production were tested in vitro and in vivo. In general, the structural and physicochemical properties, and lymphocyte proliferation activity of all samples varied with the functionalized groups and the degree of substitution. The results of immunological assays indicated that some modified derivatives had potent stimulating effects on lymphocyte proliferation and antibody production and the introduction of carboxymethyl group with low degree of substitution (DS<0.28) was the best choice on the improvement of the immunostimulating activity.
chemical modification, immunological activity, (1→3)-α-D-glucan, Ganoderma lucidum
Methods: 13C NMR, 1H NMR, GLC-MS, GLC, UV, sulfation, methylation analysis, carboxymethylation, FTIR spectroscopy, aminopropylation, hydroxyethylation, immunological assay Comments, role: data on immunological activity of chemical modifications are present
NCBI Taxonomy refs (TaxIDs):5315 Reference(s) to other database(s): GTC:G02177KX, CCSD:49417, CBank-STR:17683, GenDB:HM245773 Show glycosyltransferases
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