Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
Journal NLM ID: 0043535WWW link: http://www.sciencedirect.com/science/article/pii/S0008621502001660Publisher: Elsevier
Correspondence: jnfang
mail.shcnc.ac.cn
Institutions: Shanghai Institute of Materia Medica, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, 294 Tai-Yuan Road, Shanghai 200031, People's Republic of China, Department of Biology, Shanghai Teacher’s University, 100 Gui-Lin Road, Shanghai 200234, People’s Republic of China
A beta-D-glucan, Ab2-2N, was isolated from the hot-water extract of fruiting bodies of Agaricus blazei Murr by ethanol precipitation, anion-exchange and gel-permeation chromatography. Its structure was investigated by composition analysis, methylation analysis, Smith degradation, mild hydrolysis, and NMR spectroscopy. It contains a (1-->6)-linked beta-D-glucopyranosyl backbone, with one side chain consisting of terminal and 3-substituted beta-D-glucopyranosyl residues attached at O-3 for every three backbone residues [structure].
mushroom, β-D-glucan, Agaricus blazei, fruiting bodies
Structure type: polymer chemical repeating unit
Location inside paper: p. 1419, Ab2-2N
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, partial acid hydrolysis, TLC, acid hydrolysis, GLC, Smith degradation, HPLC, UV, methylation analysis, biological assay, MTT, ion exchange chromatography, GLC–MS, optical rotation, high-performance gel-permeation chromatography, Lowry method
Biological activity: exhibits a stimulating effect on ConA-induced T cell proliferation and LPS-induced B cell proliferation
Comments, role: proposed structure; the side chains may not be so uniformly distributed along the backbone
Related record ID(s): 50046
NCBI Taxonomy refs (TaxIDs): 79798Reference(s) to other database(s): GTC:G34338DV
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error