Cell wall and soluble polysaccharides that reacted with Trichosporon domesticum factor III serum were isolated from the type strain of T. domesticum. The fractions contained O-acetyl groups, which contributed to the serological reactivity. The antigenic structure was characterized by chromatographic and spectroscopic methods. The polysaccharide has an α-(1→3)-D-mannan backbone with hetero-oligosaccharide side chains consisting of a 2-O-substituted β-D-glucuronic acid residue bound to O-2 of the mannose residue, β-D-xylopyranosyl residues located in the middle of the side chain, and a nonreducing terminal α-L-arabinopyranosyl residue bound to 0-4 of xylose. The mannan backbone is O-acetylated at O-6 of the mannose residues

antigenic polysaccharide, chemical structures, Trichosporon domesticum

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O[C@@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](O)[C@@H](*)O[C@H](CO)[C@H]3O)O[C@H](CO)[C@H]2O)[C@H]1O[C@@H]1O[C@H](C(=O)O)[C@@H](O)[C@H](O)[C@H]1O

662.547 g/mol (C₂₄H₃₈R₂O₂₁, R = next and previous repeats, not counted in mol weight)

Cell wall and soluble polysaccharides that reacted with Trichosporon domesticum factor III serum were isolated from the type strain of T. domesticum. The fractions contained O-acetyl groups, which contributed to the serological reactivity. The antigenic structure was characterized by chromatographic and spectroscopic methods. The polysaccharide has an α-(1→3)-D-mannan backbone with hetero-oligosaccharide side chains consisting of a 2-O-substituted β-D-glucuronic acid residue bound to O-2 of the mannose residue, β-D-xylopyranosyl residues located in the middle of the side chain, and a nonreducing terminal α-L-arabinopyranosyl residue bound to 0-4 of xylose. The mannan backbone is O-acetylated at O-6 of the mannose residues

antigenic polysaccharide, chemical structures, Trichosporon domesticum

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5
4	aLArap	104.45	73.27	74.92	70.76	68.76
	aDXylp	94.61	74.01	?	?	?


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5
4	aLArap	4.38	3.54	3.64	3.92	3.63-3.94
	aDXylp	5.16	3.54	3.76	?	?


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5
4	aLArap	104.45/4.38	73.27/3.54	74.92/3.64	70.76/3.92	68.76/3.63-3.94
	aDXylp	94.61/5.16	74.01/3.54	?/3.76	?/?	?/?

The spectrum also has 3 signals at unknown positions (not plotted).

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

O[C@H]1CO[C@@H](O[C@@H]2CO[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

282.245 g/mol (C₁₀H₁₈O₉)

Cell wall and soluble polysaccharides that reacted with Trichosporon domesticum factor III serum were isolated from the type strain of T. domesticum. The fractions contained O-acetyl groups, which contributed to the serological reactivity. The antigenic structure was characterized by chromatographic and spectroscopic methods. The polysaccharide has an α-(1→3)-D-mannan backbone with hetero-oligosaccharide side chains consisting of a 2-O-substituted β-D-glucuronic acid residue bound to O-2 of the mannose residue, β-D-xylopyranosyl residues located in the middle of the side chain, and a nonreducing terminal α-L-arabinopyranosyl residue bound to 0-4 of xylose. The mannan backbone is O-acetylated at O-6 of the mannose residues

antigenic polysaccharide, chemical structures, Trichosporon domesticum

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O[C@@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](O)[C@@H](*)O[C@H](COC(C)=O)[C@H]3O)O[C@H](COC(C)=O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](C(=O)O)[C@@H](O)[C@H](O)[C@H]1O[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O[C@@H]1OC[C@@H](O[C@@H]2OC[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

1142.966 g/mol (C₄₃H₆₆R₂O₃₅, R = next and previous repeats, not counted in mol weight)

Cell wall and soluble polysaccharides that reacted with Trichosporon domesticum factor III serum were isolated from the type strain of T. domesticum. The fractions contained O-acetyl groups, which contributed to the serological reactivity. The antigenic structure was characterized by chromatographic and spectroscopic methods. The polysaccharide has an α-(1→3)-D-mannan backbone with hetero-oligosaccharide side chains consisting of a 2-O-substituted β-D-glucuronic acid residue bound to O-2 of the mannose residue, β-D-xylopyranosyl residues located in the middle of the side chain, and a nonreducing terminal α-L-arabinopyranosyl residue bound to 0-4 of xylose. The mannan backbone is O-acetylated at O-6 of the mannose residues

antigenic polysaccharide, chemical structures, Trichosporon domesticum

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5
4	aLArap	104.45	73.27	74.92	70.76	68.76
	bDXylp	99.12	76.65	76.61	79.09	65.66


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5
4	aLArap	4.38	3.54	3.64	3.92	3.63-3.94
	bDXylp	4.57	3.25	3.55	3.76	3.37-4.04


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5
4	aLArap	104.45/4.38	73.27/3.54	74.92/3.64	70.76/3.92	68.76/3.63-3.94
	bDXylp	99.12/4.57	76.65/3.25	76.61/3.55	79.09/3.76	65.66/3.37-4.04

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

O[C@H]1CO[C@@H](O[C@@H]2CO[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

282.245 g/mol (C₁₀H₁₈O₉)