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Ohno N, Uchiyama M, Tsuzuki A, Tokunaka K, Miura NN, Adachi Y, Aizawa MW, Tamura H, Tanaka S, Yadomae T
Solubilization of yeast cell-wall beta-(1-->3)-D-glucan by sodium hypochlorite oxidation and dimethyl sulfoxide extraction
Carbohydrate Research 316 (1999)
161-172
Candida albicans 1385
(Ancestor NCBI TaxID 5476,
species name lookup)
Candida albicans 1594
(Ancestor NCBI TaxID 5476,
species name lookup)
Candida albicans 0583
(Ancestor NCBI TaxID 5476,
species name lookup)
Candida parapsilosis 0640
(Ancestor NCBI TaxID 5480,
species name lookup)
Candida parapsilosis 0708
(Ancestor NCBI TaxID 5480,
species name lookup)
Candida parapsilosis 1068
(Ancestor NCBI TaxID 5480,
species name lookup)
Saccharomyces cerevisiae
(NCBI TaxID 4932,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
Publication DOI: 10.1016/S0008-6215(99)00049-XJournal NLM ID: 0043535Publisher: Elsevier
Correspondence: ohnonao
ps.toyaku.ac.jp
Institutions: Tokyo University of Pharmacy and Life Science, School of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan, Seikagaku Corporation, 3-1253 Tateno, Higashiyamato, Tokyo 207-0021, Japan
The limulus test is a well-established method for the diagnosis of both Gram-negative sepsis and invasive fungal infection. To diagnose fungal infections, a beta-(1-->3)-D-glucan-specific chromogenic kit (Fungitec G test MK) has been developed and applied clinically. We are concentrating our main efforts on developing a better standard to improve the precision of this method. To this end, we have successfully developed a protocol to obtain a soluble Candida spp. beta-(1-->3)-D-glucan (CSBG) by sodium hypochlorite (NaClO) oxidation and subsequent dimethyl sulfoxide (Me2SO) extraction (yield of 9.6 +/- 4.1%) of acetone-dried whole-cell preparations. The beta-glucan fraction is free from the cell-wall mannan, gives a symmetrical peak by gel filtration, and is soluble in dilute NaOH. The product is composed mainly of beta-(1-->3)- and beta-(1-->6)-D-glucosidic linkages. The specific activity of the beta-glucan is comparable with pachyman when combined with the Fungitec G test as the standard glucan and reacted as low as 10(-11) g/mL.
cell wall, MALDIMS, β-glucan, Candida spp., limulus test
Structure type: structural motif or average structure
Location inside paper: GSBG (fig. 8)
Trivial name: β-1,3-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, ELISA, antitumor activity assay, MALDI-TOF-MS, phenol-sulfuric acid method, capillary GLC, limulus test, NaClO oxidation, Me2SO extraction, zymolyase digestion
Biological activity: immunomodulating activity; antitumor activity
Comments, role: part of suggested beta-(1->3)/(1->6) structure
Related record ID(s): 40623
NCBI Taxonomy refs (TaxIDs): 5476,
5480,
4932Reference(s) to other database(s): GTC:G51056AN
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There is only one chemically distinct structure:
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Ohno N, Uchiyama M, Tsuzuki A, Tokunaka K, Miura NN, Adachi Y, Aizawa MW, Tamura H, Tanaka S, Yadomae T
Solubilization of yeast cell-wall beta-(1-->3)-D-glucan by sodium hypochlorite oxidation and dimethyl sulfoxide extraction
Carbohydrate Research 316 (1999)
161-172
Candida albicans 1385
(Ancestor NCBI TaxID 5476,
species name lookup)
Candida albicans 1594
(Ancestor NCBI TaxID 5476,
species name lookup)
Candida albicans 0583
(Ancestor NCBI TaxID 5476,
species name lookup)
Candida parapsilosis 0640
(Ancestor NCBI TaxID 5480,
species name lookup)
Candida parapsilosis 0708
(Ancestor NCBI TaxID 5480,
species name lookup)
Candida parapsilosis 1068
(Ancestor NCBI TaxID 5480,
species name lookup)
Saccharomyces cerevisiae
(NCBI TaxID 4932,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
Publication DOI: 10.1016/S0008-6215(99)00049-XJournal NLM ID: 0043535Publisher: Elsevier
Correspondence: ohnonao
ps.toyaku.ac.jp
Institutions: Tokyo University of Pharmacy and Life Science, School of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan, Seikagaku Corporation, 3-1253 Tateno, Higashiyamato, Tokyo 207-0021, Japan
The limulus test is a well-established method for the diagnosis of both Gram-negative sepsis and invasive fungal infection. To diagnose fungal infections, a beta-(1-->3)-D-glucan-specific chromogenic kit (Fungitec G test MK) has been developed and applied clinically. We are concentrating our main efforts on developing a better standard to improve the precision of this method. To this end, we have successfully developed a protocol to obtain a soluble Candida spp. beta-(1-->3)-D-glucan (CSBG) by sodium hypochlorite (NaClO) oxidation and subsequent dimethyl sulfoxide (Me2SO) extraction (yield of 9.6 +/- 4.1%) of acetone-dried whole-cell preparations. The beta-glucan fraction is free from the cell-wall mannan, gives a symmetrical peak by gel filtration, and is soluble in dilute NaOH. The product is composed mainly of beta-(1-->3)- and beta-(1-->6)-D-glucosidic linkages. The specific activity of the beta-glucan is comparable with pachyman when combined with the Fungitec G test as the standard glucan and reacted as low as 10(-11) g/mL.
cell wall, MALDIMS, β-glucan, Candida spp., limulus test
Structure type: structural motif or average structure
Location inside paper: GSBG (fig. 8)
Trivial name: β-1,6-glucan
Compound class: EPS, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, ELISA, antitumor activity assay, MALDI-TOF-MS, phenol-sulfuric acid method, capillary GLC, limulus test, NaClO oxidation, Me2SO extraction, zymolyase digestion
Biological activity: immunomodulating activity; antitumor activity
Comments, role: part of suggested beta-(1->3)/(1->6) structure
Related record ID(s): 40446
NCBI Taxonomy refs (TaxIDs): 5476,
5480,
4932Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
There is only one chemically distinct structure:
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