CSDB: Search results

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

Publication DOI: 10.1046/j.1365-313x.2000.00792.x
Journal NLM ID: 9207397
Publisher: Oxford: Blackwell Scientific Publishers and BIOS Scientific Publishers for the Society for Experimental Biology
Correspondence: narsimhan

hort.purdue.edu
Institutions: Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, IN 47907-1165, USA, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, PO Box 1052, Sevilla, E-41080, Spain

Osmotin is a plant PR-5 protein. It has a broad spectrum of antifungal activity, yet also exhibits specificity for certain fungal targets. The structural bases for this specificity remain unknown. We show here that full sensitivity of Saccharomyces cerevisiae cells to the PR-5 protein osmotin is dependent on the function of MNN2, MNN4 and MNN6. MNN2 is an α-1,2-mannosyltransferase catalyzing the addition of the first mannose to the branches on the poly l,6-mannose backbone of the outer chain of cell wall N-linked mannans. MNN4 and MNN6 are required for the transfer of mannosylphosphate to cell wall mannans. Null mnn2, mnn4 or mnn6 mutants lack phosphomannans and are defective in binding osmotin to the fungal cell wall. Both antimannoprotein antibody and the cationic dye alcian blue protect cells against osmotin cytotoxicity. MNN1 is an α-1,3-mannosyltransferase that adds the terminal mannose to the outer chain branches of N-linked mannan, masking mannosylphosphate. Null mnn1 cells exhibit enhanced osmotin binding and sensitivity. Several cell wall mannoproteins can bind to immobilized osmotin, suggesting that their polysaccharide constituent determines osmotin binding. Our results demonstrating a causal relationship between cell surface phosphomannan and the susceptibility of a yeast strain to osmotin suggest that cell surface polysaccharides of invading pathogens control target specificity of plant PR-5 proteins.

cell wall, mannan, fungal, Saccharomyces cerevisiae, pathogenesis-related protein, thamatin-like

Structure type: structural motif or average structure
Location inside paper: fig. 1
Compound class: N-glycan
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_1394182,IEDB_140116,IEDB_141111,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_141832,IEDB_143632,IEDB_144983,IEDB_144996,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_174840,IEDB_241100,IEDB_474450,IEDB_76933,IEDB_857732,IEDB_857734,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_44,SB_53,SB_55,SB_67,SB_72,SB_73,SB_77

Methods: SDS-PAGE, biological assays, binding assays, Edman degradation, genetic analysis
Synthetic data: enzymatic

Related record ID(s): 40792, 40793
NCBI Taxonomy refs (TaxIDs): 4932
Show glycosyltransferases

There are too many chemically distinct structures (~6.6x10²⁰), so only one is shown:

aDManp(1-3)aDManp(1-2)aDManp(1-2)[aDManp(1-2)[aDManp(1-3)aDManp(1-2)[aDManp(1-3)aDManp(1-P-3)]aDManp(1-2)[aDManp(1-3)aDManp(1-2)aDManp(1-2)[aDManp(1-3)aDManp(1-2)aDManp(1-2)[aDManp(1-2)aDManp(1-2)[aDManp(1-2)aDManp(1-6)]aDManp(1-6)]aDManp(1-6)]aDManp(1-6)]aDManp(1-6)]aDManp(1-6)]aDManp(1-3)[aDManp(1-3)[aDManp(1-3)aDManp(1-2)aDManp(1-6)]aDManp(1-6)]aDManp(1-4)[Ac(1-2)]?DGlcpN(1-3)[Ac(1-2)]?DGlcpN1N(1-4)x?Asn?

CC(=O)N[C@H]1C(O[C@H]2[C@H](O)[C@@H](CO)OC(NC(=O)CC(N)C(=O)O)[C@@H]2NC(C)=O)O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@@H](O)[C@H](O[C@H]3O[C@H](CO[C@H]4O[C@H](CO[C@H]5O[C@H](CO[C@H]6O[C@H](CO[C@H]7O[C@H](CO[C@H]8O[C@H](CO[C@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]9O[C@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]9O)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O)[C@@H]7O)[C@@H](O)[C@H](O)[C@@H]6O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]6O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O)[C@@H]6O)[C@@H](O)[C@H](O)[C@@H]5O[C@H]5O[C@H](CO)[C@@H](O)[C@H](OP(=O)(O)O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O)[C@@H]6O)[C@@H]5O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]6O)[C@@H]5O)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

cell wall, mannan, fungal, Saccharomyces cerevisiae, pathogenesis-related protein, thamatin-like

Structure type: structural motif or average structure
Location inside paper: fig. 1
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_137485,IEDB_1394182,IEDB_140116,IEDB_141111,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_144996,IEDB_150900,IEDB_152206,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_174840,IEDB_474450,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72

Methods: SDS-PAGE, biological assays, binding assays, Edman degradation, genetic analysis
Synthetic data: enzymatic

Related record ID(s): 40536, 40793
NCBI Taxonomy refs (TaxIDs): 4932
Show glycosyltransferases

There are too many chemically distinct structures (~12288), so only one is shown:

aDManp(1-3)aDManp(1-3)aDManp(1-2)[?DManp(1-P-3)]aDManp(1-2)aDManp(1-3)x?Ser?

NC(CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O)[C@H](OP(=O)(O)OC2O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]2O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@@H]2O)[C@@H]1O)C(=O)O

SVG MW SMILES 3D mol Ionize

cell wall, mannan, fungal, Saccharomyces cerevisiae, pathogenesis-related protein, thamatin-like

Structure type: structural motif or average structure
Location inside paper: fig. 1
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_137485,IEDB_1394182,IEDB_140116,IEDB_141111,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_144996,IEDB_152206,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_174840,IEDB_474450,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72

Methods: SDS-PAGE, biological assays, binding assays, Edman degradation, genetic analysis
Synthetic data: enzymatic

Related record ID(s): 40536, 40792
NCBI Taxonomy refs (TaxIDs): 4932
Show glycosyltransferases

There are too many chemically distinct structures (~24576), so only one is shown:

aDManp(1-3)aDManp(1-3)aDManp(1-2)[?DManp(1-P-3)]aDManp(1-2)aDManp(1-3)xDThr?

C[C@H](O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O)[C@H](OP(=O)(O)OC2O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]2O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@@H]2O)[C@@H]1O)[C@@H](N)C(=O)O

SVG MW SMILES 3D mol Ionize