Taxonomic group: fungi / Basidiomycota, Ascomycota
(Phylum: Basidiomycota, Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 34560984Publication DOI: 10.1016/j.carbpol.2021.118582Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Jianrong Wu <kinowu
jiangnan.edu.cn>
Institutions: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
The large molecular weight and poor water solubility of β-1,3-glucan impede its potential applications. In this study, the β-1,3-glucan producing fungi and Trichoderma harzianum capable of secreting endo-β-1,3-glucanase were co-cultivated to produce branched β-1,3-glucan oligosaccharides (bOβGs) by fermentation with Sclerotium rolfsii and Schizophyllum commune. The highest bOβG yields from S. rolfsii in flasks were 4.53 and 9.94 g/L in a 7 L fermenter. Structural analysis proved that bOβG from S. rolfsii had a narrow degree of polymerization of 5-12, whereas bOβG from S. commune had a degree of polymerization of 5-15. Antioxidant tests showed that both bOβGs had remarkable DPPH radical scavenging activity and hydroxyl radical scavenging activity, and the activity of bOβG from S. commune was better than that of bOβG from S. rolfsii. In addition, bOβGs could promote the secretion of NO by mouse macrophages and increase the production of TNF-α, IL-1β, and IL-6 in RAW264.7.
Antioxidant activity, fungi, Schizophyllum commune, Trichoderma harzianum, co-cultivation, branched β-1, 3-glucan oligosaccharide
Structure type: fragment of a bigger structure
Location inside paper: p. 118582-4
Trivial name: β-1,3-glucan, botryosphaeran, β-glucan
Compound class: EPS, cell wall polysaccharide, β-gucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 1H NMR, acid hydrolysis, MALDI-TOF MS, HPLC, extraction, enzymatic assay, cytokine production, antioxidant activity assay, cultivation methods, co-cultivation
Comments, role: bOβGs (branched β-1,3-glucan oligosaccharide) prepared from co-cultivation of T. harzianum with S. rolfsii
NCBI Taxonomy refs (TaxIDs): 39291,
5544Reference(s) to other database(s): GTC:G83138WS
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There is only one chemically distinct structure:
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(later renamed to: Athelia rolfsii)