Bacteria belonging to the genus Aeromonas are Gram-negative mesophilic and essentially ubiquitous in the microbial biosphere; moreover they are considered very important pathogens in fish and responsible for a great variety of human infections. The virulence of Gram-negative bacteria is often associated with the structure of lipopolysaccharides, which consist of three regions covalently linked: the glycolipid (lipid A), the oligosaccharide region (core region) and the O-specific polysaccharide (O-chain, O-antigen). The O-chain region seems to play an important role in host-pathogen interaction. In the case of Aeromonas hydrophila the majority of pathogenic strains belongs to serogroups O:11, O:16, O:18 and O:34. In this paper, we report the complete structure of the O-chain of A. hydrophila strain A19 (serogroup O:14), a pathogenic strain isolated from European eels, which showed high virulence when tested in trout or mice. Dried cells were extracted by the PCP (phenol/chloroform/petroleum ether) method obtaining the lipopolysaccharide. After mild acid hydrolysis the lipid A was removed by centrifugation and the obtained polysaccharide was fully characterized by means of chemical analysis and one- and two-dimensional NMR spectroscopy. All the data collected are directed towards the following structure: (formula: see text).

Lipopolysaccharide, NMR spectroscopy, structure elucidation, Aeromonas

• Compound ID: 9772
  

  a-D-Glcp-(1-2)-+ | -3)-a-L-Rhap-(1-4)-a-D-Glcp-(1-

  Show graphically

  Show legend Show as text

  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide, O-antigen
  Reference(s) to other database(s): GTC:G54049LH
  
   
  
  Aeromonas hydrophila O14 (A19)
  CSDB ID 26317 (all data & tools)