Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting bodies
 
The structure was elucidated in this paper
NCBI PubMed ID: 33476617
Publication DOI: 10.1016/j.ijbiomac.2021.01.093
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: Hai-Xue Kuang <hxkuang56163.com>; Yong-Gang Xia <yonggangxia163.com>
Institutions: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin, China

In this study, an uncommon enzymatic-fingerprinting workflow, was proposed for characterization and discrimination of mushroom polysaccharides (MPs) by hydrophilic interaction liquid chromatography-negative electrospray mass spectrometry (HILIC-ESI--MS). Firstly, the HILIC-ESI--MS was used to screen and identify the enzymatic digestion products of MPs using HILIC-Orbitrap based on full scan and MS/MS modes. Secondly, a targeted structural-fingerprinting of polysaccharides (SFP) was built in a multiple-ion monitoring (MIM) mode using the same HILIC separation with a triple quadrupole MS. Thirdly, a case study of polysaccharides in Hericium erinaceus fruiting bodies (HEP) was performed to obtain the expected SFP based on dextranase digestion that allows for visual discrimination of polysaccharides from other five edible mushrooms attributed to Agrocybe cylindracea, Arimillaria mellea, Flammulina velutipes, Pleurotus eryngii, and Lentinula edodes. Furthermore, a major structural backbone of HEP was unveiled by occurrence of >6(Hex)1> along with multiple possible substitutions including of terminal GalA, Fuc, acetyl, >4Hex1>, and >3Hex1>. Finally, the similarity analysis, hierarchical cluster analysis (HCA), and partial least squares discriminant analysis (PLS-DA) were performed to visualize various MPs. As a result, the enzymatic-fingerprinting workflow presents an effective example for quality evaluation of fungi polysaccharides using a SFP strategy.

medicine, Hericium erinaceus, enzymatic digestion, HILIC-ESI(-)-MS, HILIC-MIM-MS, structural-fingerprinting of polysaccharides

Structure type: structural motif or average structure
Location inside paper: p. 499, Fig. 5
Trivial name: HEP, Hericium erinaceus polysaccharides
Contained glycoepitopes: IEDB_136045,IEDB_142489,IEDB_144562,IEDB_152214,IEDB_174333,SB_86
 
Methods: methylation, GC-MS, MS/MS, enzymatic digestion, HILIC-ESI-MS, UHPLC, screening, HILIC-MIM-MS
Comments, role: The hypothetical structural skeleton and branched chains of HEP.
 
NCBI Taxonomy refs (TaxIDs): 91752
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