CSDB: Search results

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
NCBI PubMed ID: 38101321
Publication DOI: 10.1021/acs.jafc.3c05430
Journal NLM ID: 0374755
Publisher: American Chemical Society
Correspondence: R.P. Li <lirongpeng

jsnu.edu.cn>; B. Yuan <boyuan

jsnu.edu.cn>
Institutions: School of Life Science, Jiangsu Normal University, Xuzhou 221116, China

Soil salinity is an important limiting factor in agricultural production. Rhizospheric fungi can potentially enhance crop salinity tolerance, but the precise role of signaling substances is still to be systematically elucidated. A rhizospheric fungus identified as Paecilomyces vaniformisi was found to enhance the salinity tolerance of rice seedlings. In this study, a novel polysaccharide (PPL2b) was isolated from P. vaniformisi and identified as consisting of Manp, Glcp, GalpA, and Galp. In a further study, PPL2b showed significant activity in alleviating salinity stress-induced growth inhibition in rice seedlings. The results indicated that under salinity stress, PPL2b enhances seed germination, plant growth (height and biomass), and biochemical parameters (soluble sugar and protein contents). Additionally, PPL2b regulates genes such as SOS1 and SKOR to decrease K+ efflux and increase Na+ efflux. PPL2b increased the expression and activity of genes related to antioxidant enzymes and nonenzyme substances in salinity-induced oxidative stress. Further study indicated that PPL2b plays a crucial role in regulating osmotic substances, such as proline and betaine, in maintaining the osmotic balance. It also modulates plant hormones to promote rice seedling growth and enhance their tolerance to soil salinity. The variables interacted and were divided into two groups (PC1 77.39% and PC2 18.77%) based on their relative values. Therefore, these findings indicate that PPL2b from P. vaniformisi can alleviate the inhibitory effects of salinity stress on root development, osmotic adjustment, ion balance, oxidative stress balance, and growth of rice seedlings. Furthermore, it suggests that polysaccharides produced by rhizospheric fungi could be utilized to enhance crop tolerance to salinity.

polysaccharides, Antioxidant, salt tolerance, rhizospheric fungi, rice seedling

Structure type: structural motif or average structure ; 63010
Location inside paper: p. 20589, Fig. 2, table 2, PPL2b
The structure in this paper was incorrect:

Original structure from CSDB record 41047

← originally published structure

Show legend

Trivial name: polysaccharide PPL2b

Methods: 13C NMR, 1H NMR, NMR-2D, methylation, partial acid hydrolysis, GC-MS, sugar analysis, GC, biological assays, FTIR, UV, extraction, statistical analysis, antioxidant activities, HPGPC, salt-stress
Comments, role: the erroneous structure was assumed / generated by CSDB staff, as published structure has multiple contradictions with text and NMR data; published NMR spectra were removed by CSDB staff due to multiple errors in the NMR assignment.

NCBI Taxonomy refs (TaxIDs): 33202
Show glycosyltransferases

NMR conditions: in D2O

¹H NMR data: present in publication with errors

¹³C NMR data: present in publication with errors

There are too many chemically distinct structures (~24576), so only one is shown:

-4)bDGalpA(1-2)SUG(1-4)[a?Sugp(1-6)]bDManp(1-3)aDManp(1-6)[aDGalp(1-4)]bDGlcp(1-6)[aDGalp(1-4)]bDManp(1-4)bDGlcp(1-3)[a?Sugp(1-2)]bDManp(1-4)[a?Sugp(1-6)]bDGlcp(1-2)SUG(1-4)bDGalpA(1- // Sug = Glcp or Galp linked by (1-4) or (1-6) glycosidic bond to b?Manp

*O[C@H]1[C@@H](C(=O)O)O[C@@H](O[1*]O[C@@H]2[C@@H](CO[2*])O[C@@H](O[C@@H]3[C@H](O)[C@@H](OC[C@H]4O[C@@H](OC[C@H]5O[C@@H](O[C@@H]6[C@@H](CO)O[C@@H](O[C@@H]7[C@H](O[2*])[C@H](O[C@@H]8[C@@H](CO[2*])O[C@@H](O[1*]O[C@H]9[C@@H](C(=O)O)O[C@@H](*)[C@H](O)[C@H]9O)[C@H](O)[C@H]8O)O[C@H](CO)[C@H]7O)[C@H](O)[C@H]6O)[C@@H](O)[C@@H](O)[C@@H]5O[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H](CO)[C@H]3O)[C@@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

R1 = SUG
R2 = a?Sugp, Sug = Glcp or Galp linked by (1-4) or (1-6) glycosidic bond to b?Manp