Li Z, Wang M, Yang Z Structural characterization, anti-tumor and immunomodulatory activity of intracellular polysaccharide from Armillaria luteo-virens Carbohydrate Research534 (2023)
108945
The structure was elucidated in this paper NCBI PubMed ID:37738818 Publication DOI:10.1016/j.carres.2023.108945 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: M. Wang <wangmiaosichuan163.com> Institutions: Laboratory of Molecular Oncology, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610064, China, Laboratory Animal Center, West China School of Basic Medical Science & Forensic Medicine, Sichuan University, Chengdu, 610041, China, Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China
Armillaria luteo-virens (A. luteo-virens) is a kind of edible fungus mainly exists in Qinghai-Tibet of China, but at present only very few studies focus on the bioactivities of its polysaccharides. This study aimed to purify and characterize the structure features of a novel intracellular polysaccharide (ALP-A) derived from A. luteo-virens and explore its potential anti-tumor and immunomodulatory activities. Through systematic separation and purification, we obtained a homogeneous ALP-A with an average molecular weight of 23693Da. Structural analysis indicated that ALP-A was mainly composed of glucose and mannose with a molar ratio of 6.02:1. The repeating unit of ALP-A was →4)-α-D-Glcp-(1→ backbone with α-Glcp-(1→ and α-Manp-(6→ side chains which branched at O-2 position. The anti-tumor assays in vivo suggested that ALP-A could effectively restrain S180 solid tumor growth, protect immune organs and promote the secretion of cytokines (IL2, IL6 and TNF-α) in serum. Besides, in vitro immunomodulatory assays indicated that ALP-A could improve proliferation, phagocytic capacity and raise the level of NO and cytokines in Raw264.7 cells. These results demonstrate that ALP-A which possess potential antitumor and immunomodulatory abilities can be developed as a new functional food.
Methods: 1H NMR, methylation, GC-MS, sugar analysis, FTIR, HPLC, cytokine analysis, extraction, statistical analysis, cell viability assay, HPGPC, antitumor activity assay, immunomodulatory activity analysis, animal experiment Biological activity: ALP-A exhibited antitumor abilities in vivo and vitro Comments, role: Putative structure of ALP-A; error in the paper: aDManp forms an outgoing bond at position 6 (not at C1) in Fig. 7.
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(later renamed to: Floccularia luteovirens)
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