Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperNCBI PubMed ID: 37659785Publication DOI: 10.1016/j.carbpol.2023.121176Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: A.P. Vanin <apvanin
ucs.br>
Institutions: Enzymes and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil, Nuclear Magnetic Resonance Laboratory, Federal University of São Carlos, São Carlos, SP, Brazil, Computational Biology and Bioinformatics Laboratory, Bioinformatics Research Center, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil, Biophysics and Medical Technology, Department of Physics, NTNU Norwegian University of Science and Technology, Trondheim, Norway, Institute of Chemistry, Federal University of Catalao, Catalao, GO, Brazil
A co-metabolization of xylose and glucose by Schizophyllum commune 227E.32 wild mushroom for exopolysaccharide (EPS) production is presented. Cultivations performed with S. commune 227E.32 at different xylose concentrations demonstrated that the concentration of 50 g·L-1 of xylose achieved the highest EPS production, around 4.46 g·L-1. Scale-up in a stirred tank reactor (STR) was performed. 10 % inoculum showed the highest cost/benefit ratio regarding sugar conversion and EPS production (Y P/S = 0.90 g·g-1), achieving 1.82 g·L-1 of EPS. Isolation, purification, and characterization were conducted with EPS produced in flasks and STR. GC-MS analysis showed glucose as main monosaccharide constituents for both isolates. 13C NMR and HSQC-edited showed that both EPS isolated consisted of a β-D-Glcp (1→3) main chain, partially substituted at O-6 with nonreducing β-D-Glcp ends on every third residue, similar to β-D-glucan isolated from S. commune basidiomes known as schizophyllan (SPG). The Mw was determined by GPC to 1.5 × 106 Da (flasks) and 1.1 × 106 Da (STR). AFM topographs revealed a semi-flexible appearance of the β-D-glucan, consistent with the triple helical structures adopted by SPG and overall contour length consistent with a high molar mass.
physicochemical characterization, mushroom β-(1 → 3)(1 → 6)glucan, xylose/glucose co-metabolization
Structure type: polymer chemical repeating unit ; 1100000-1500000
Location inside paper: abstract, Fig. 6(c)
Trivial name: schizophyllan (SPG)
Compound class: EPS
Methods: 13C NMR, 1H NMR, methylation, GC-MS, GPC, UV, viscosity measurement, AFM, isolation, EPS production, cultivations
Comments, role: published polymerization frame was shifted for conformity with other records
NCBI Taxonomy refs (TaxIDs): 5334
Show glycosyltransferases
There is only one chemically distinct structure:
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