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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: mycelium

The structure was elucidated in this paper
NCBI PubMed ID: 36596100
Publication DOI: 10.1016/j.foodres.2022.112146
Journal NLM ID: 9210143
Publisher: Ottawa, Ontario, Canada: CIFST, Elsevier Applied Science
Correspondence: Z. Zhu <zhyuanzhu

tust.edu.cn>
Institutions: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China, Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China, College of Public Health, Zunyi Medical University, Guizhou 563006, PR China

Pleurotus ostreatus is one of the most common edible and medicinal fungi in life, and its polysaccharide has been a hot research topic in recent years. In this paper, a new intracellular polysaccharide component named P. ostreatus polysaccharide (POP-W) was obtained from the mycelium of P. ostreatus, and its structure was analyzed. The results showed that its molecular weight was Mw = 3.034 × 103 kDa, and it did not contain protein and nucleic acid. POP-W was composed of mannose, glucose, galactose and xylose in a molar ratio of 40.34:47.60:7.97:4.09. The backbone of POP-W was α-D-Glcp(1→,→3,4)-α-D-Glcp(1→, →3,4)-α-D-Manp(1→,→3)-α -D-Galp(1→, →4)-α-D-Glcp(1→, →3)-α-D-Glcp(1→, →2)-β-D-Manp(1→, →4)-β-D-Xylp(1→. SEM and TGA analysis showed the structure of POP-W and good thermal stability. In addition, POP-W showed significant antioxidant activity in vitro. More importantly, POP-W protected PC12 cells induced by H2O2 by inhibiting the contents of lactate dehydrogenase (LDH) and malondialdehyde (MDA) and increasing the levels of superoxide dismutase (SOD) and reduced glutathione (GSH). Western blot detection of Caspase-3, BAX, Bcl-2, PI3K/Akt protein expression. The results showed that POP-W inhibited the expression of caspase-3 and BAX, while promoting the expression of Bcl-2. In addition, POP-W can also promote the phosphorylation of Akt. In conclusion, POP-W pretreatment can protect PC12 cells from H2O2-induced oxidative damage through PI3K/Akt signaling pathway and regulation of apoptosis-related pathway proteins. It provided a theoretical basis for the practical application of the polysaccharide of P. ostreatus in production.

polysaccharide, Structural characterization, Antioxidant, PC12 cells, P.ostreatus

Structure type: structural motif or average structure ; 3034
Location inside paper: table 2, Fig. 4(g), POP-W
Trivial name: polysaccharide (POP-W)
Compound class: IPS

Methods: 13C NMR, 1H NMR, methylation, GC-MS, Smith degradation, Western blotting, FTIR, composition analysis, HPLC, extraction, periodate oxidation, statistical analysis, antioxidant activities, cell viability assay, radical scavenging assay, SEM, TGA, isolation, oxidative stress, SOD, GSH, MDA
Comments, role: NMR temperature was not specified; published erroneous NMR chemical shifts of C2 aDGalp (76.05) was removed by CSDB staff.

NCBI Taxonomy refs (TaxIDs): 5322
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4,4,4,3,3,4	aDGlcp	100.17	72.45	74.13	76.84	73.20	61.18
4,4,4,4,3	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,4,4,3,2	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,3	aDManp	99.30	78.65	71.72	65.49	69.68	60.46
4,4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	101.64	71.62	82.20	69.58	75.46	62.19
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	102.37	74.43	74.48	76.80	63.57
4,4,4,4	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
	SUG


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4,4,4,3,3,4	aDGlcp	4.94	3.37	3.48	3.56	3.51	3.63
4,4,4,4,3	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,4,4,3,2	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,3	aDManp	5.29	3.75	3.84	3.69	3.96	3.69
4,4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	4.57	3.61	3.71	4.02	3.65	3.80
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	4.43	3.15	3.40	3.58	3.20
4,4,4,4	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
	SUG


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,4,4,4,3,3,4	aDGlcp	100.17/4.94	72.45/3.37	74.13/3.48	76.84/3.56	73.20/3.51	61.18/3.63
4,4,4,4,3	aDGalp	101.79/4.99	?/3.84	81.52/4.02	69.27/3.96	75.26/3.52	61.30/3.73
4,4,4,3,2	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,4,4,3	aDManp	99.30/5.29	78.65/3.75	71.72/3.84	65.49/3.69	69.68/3.96	60.46/3.69
4,4,4	aDManp	101.25/5.19	71.59/3.62	81.29/3.92	76.41/3.82	72.86/3.76	62.73/3.74
4,4,3,3	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,4,3	aDManp	102.12/5.03	69.54/4.22	77.94/3.95	70.84/3.74	73.14/3.71	60.89/3.75
4,4	aDManp	101.25/5.19	71.59/3.62	81.29/3.92	76.41/3.82	72.86/3.76	62.73/3.74
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	101.64/4.57	71.62/3.61	82.20/3.71	69.58/4.02	75.46/3.65	62.19/3.80
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	102.37/4.43	74.43/3.15	74.48/3.40	76.80/3.58	63.57/3.20
4,4,4,4	aDGalp	101.79/4.99	?/3.84	81.52/4.02	69.27/3.96	75.26/3.52	61.30/3.73
4,3,3	aDGlcp	100.10/4.89	70.93/3.66	75.53/3.72	70.91/3.43	73.89/3.82	60.99/3.74
4,3	aDManp	102.12/5.03	69.54/4.22	77.94/3.95	70.84/3.74	73.14/3.71	60.89/3.75
4	aDManp	101.25/5.19	71.59/3.62	81.29/3.92	76.41/3.82	72.86/3.76	62.73/3.74
	SUG

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4,4,4,3,3,4	aDGlcp	4.94	3.37	3.48	3.56	3.51	3.63
4,4,4,4,3	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,4,4,3,2	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,3	aDManp	5.29	3.75	3.84	3.69	3.96	3.69
4,4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4,4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	4.57	3.61	3.71	4.02	3.65	3.80
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	4.43	3.15	3.40	3.58	3.20
4,4,4,4	aDGalp	4.99	3.84	4.02	3.96	3.52	3.73
4,3,3	aDGlcp	4.89	3.66	3.72	3.43	3.82	3.74
4,3	aDManp	5.03	4.22	3.95	3.74	3.71	3.75
4	aDManp	5.19	3.62	3.92	3.82	3.76	3.74
	SUG

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
4,4,4,4,3,3,4	aDGlcp	100.17	72.45	74.13	76.84	73.20	61.18
4,4,4,4,3	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,4,4,3,2	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,3	aDManp	99.30	78.65	71.72	65.49	69.68	60.46
4,4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4,4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
4,4,4,4,3,3,4,4,3,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,4,4,4,3,3,4,4,3,3	bDGlcp
4,4,4,4,3,3,4,4,3	bDGlcp	101.64	71.62	82.20	69.58	75.46	62.19
4,4,4,4,3,3,4,4	bDGlcp
4,4,4,4,3,3	bDXylp	102.37	74.43	74.48	76.80	63.57
4,4,4,4	aDGalp	101.79	?	81.52	69.27	75.26	61.30
4,3,3	aDGlcp	100.10	70.93	75.53	70.91	73.89	60.99
4,3	aDManp	102.12	69.54	77.94	70.84	73.14	60.89
4	aDManp	101.25	71.59	81.29	76.41	72.86	62.73
	SUG

The spectrum also has 2 signals at unknown positions (not plotted).

SMILES errors: aDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-4)/aDGlcp(1-4)/n=8/bDXylp(1-3)/aDGalp(1-3)/n=3/aDGalp(1-4)/[aDGlcp(1-2)aDManp(1-3)]aDManp(1-4)/n=2//[aDGlcp(1-3)aDManp(1-3)]aDManp(1-4)/n=4/[aDGlcp(1-3)aDManp(1-3)]aDManp(1-4)SUG:
SMILES error: could not calculate brutto descriptors of a molecule from SMILES [1*]O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O[C@H]6O[C@H](CO)[C@@H](O[C@H]7O[C@H](CO)[C@@H](O[C@H]8O[C@H](CO)[C@H](O)[C@H](O[C@H]9O[C@H](CO)[C@H](O)[C@H](O[C@H]%10O[C@H](CO)[C@H](O)[C@H](O[C@H]%11O[C@H](CO)[C@H](O)[C@H](O[C@@H]%12OC[C@@H](O[C@H]%13O[C@H](CO)[C@@H](O[C@H]%14O[C@H](CO)[C@@H](O[C@H]%15O[C@H](CO)[C@@H](O[C@H]%16O[C@H](CO)[C@@H](O[C@H]%17O[C@H](CO)[C@@H](O[C@@H]%18O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%19O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%20O[C@H](CO)[C@@H](O)[C@H](O[C@H]%21O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%21O)[C@H]%20O)[C@H]%19O)[C@H]%18O)[C@H](O)[C@H]%17O)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@H](O)[C@H]%14O)[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H]%11O)[C@H]%10O)[C@H]9O)[C@H]8O)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H]7O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@@H]5O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H]2O)[C@@H]1O
Invalid SMILES [1*]O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O[C@H]6O[C@H](CO)[C@@H](O[C@H]7O[C@H](CO)[C@@H](O[C@H]8O[C@H](CO)[C@H](O)[C@H](O[C@H]9O[C@H](CO)[C@H](O)[C@H](O[C@H]%10O[C@H](CO)[C@H](O)[C@H](O[C@H]%11O[C@H](CO)[C@H](O)[C@H](O[C@@H]%12OC[C@@H](O[C@H]%13O[C@H](CO)[C@@H](O[C@H]%14O[C@H](CO)[C@@H](O[C@H]%15O[C@H](CO)[C@@H](O[C@H]%16O[C@H](CO)[C@@H](O[C@H]%17O[C@H](CO)[C@@H](O[C@@H]%18O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%19O[C@H](CO)[C@@H](O)[C@H](O[C@@H] O[C@H](CO)[C@@H](O)[C@H](O[C@H]%21O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%21O)[C@H] O)[C@H]%19O)[C@H]%18O)[C@H](O)[C@H]%17O)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@H](O)[C@H]%14O)[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H]%11O)[C@H]%10O)[C@H]9O)[C@H]8O)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H]7O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@@H]5O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H]2O)[C@@H]1O