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1. (CSDB ID: 41094) | report error |
| a-D-Glcp-(1-3)-a-D-Manp-(1-3)-+ | a-D-Glcp-(1-3)-a-D-Manp-(1-3)-+ | | | a-D-Glcp-(1-2)-a-D-Manp-(1-3)-+ | | | | | a-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-4)-{{{-a-D-Glcp-(1-4)-}}}/n=8/-b-D-Xylp-(1-3)-{{{-a-D-Galp-(1-3)-}}}/n=3/-a-D-Galp-(1-4)-{{{-a-D-Manp-(1-4)-}}}/n=2/-{{{-a-D-Manp-(1-4)-}}}/n=4/-a-D-Manp-(1-4)-SUG | Show graphically |
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Pleurotus ostreatus
(NCBI TaxID 5322,
species name lookup)
tust.edu.cn>Pleurotus ostreatus is one of the most common edible and medicinal fungi in life, and its polysaccharide has been a hot research topic in recent years. In this paper, a new intracellular polysaccharide component named P. ostreatus polysaccharide (POP-W) was obtained from the mycelium of P. ostreatus, and its structure was analyzed. The results showed that its molecular weight was Mw = 3.034 × 103 kDa, and it did not contain protein and nucleic acid. POP-W was composed of mannose, glucose, galactose and xylose in a molar ratio of 40.34:47.60:7.97:4.09. The backbone of POP-W was α-D-Glcp(1→,→3,4)-α-D-Glcp(1→, →3,4)-α-D-Manp(1→,→3)-α -D-Galp(1→, →4)-α-D-Glcp(1→, →3)-α-D-Glcp(1→, →2)-β-D-Manp(1→, →4)-β-D-Xylp(1→. SEM and TGA analysis showed the structure of POP-W and good thermal stability. In addition, POP-W showed significant antioxidant activity in vitro. More importantly, POP-W protected PC12 cells induced by H2O2 by inhibiting the contents of lactate dehydrogenase (LDH) and malondialdehyde (MDA) and increasing the levels of superoxide dismutase (SOD) and reduced glutathione (GSH). Western blot detection of Caspase-3, BAX, Bcl-2, PI3K/Akt protein expression. The results showed that POP-W inhibited the expression of caspase-3 and BAX, while promoting the expression of Bcl-2. In addition, POP-W can also promote the phosphorylation of Akt. In conclusion, POP-W pretreatment can protect PC12 cells from H2O2-induced oxidative damage through PI3K/Akt signaling pathway and regulation of apoptosis-related pathway proteins. It provided a theoretical basis for the practical application of the polysaccharide of P. ostreatus in production.
polysaccharide, Structural characterization, Antioxidant, PC12 cells, P.ostreatus
Structure type: structural motif or average structure ; 303413C NMR data: Linkage Residue C1 C2 C3 C4 C5 C6 4,4,4,4,3,3,4 aDGlcp 100.17 72.45 74.13 76.84 73.20 61.18 4,4,4,4,3 aDGalp 101.79 ? 81.52 69.27 75.26 61.30 4,4,4,3,2 aDGlcp 100.10 70.93 75.53 70.91 73.89 60.99 4,4,4,3 aDManp 99.30 78.65 71.72 65.49 69.68 60.46 4,4,4 aDManp 101.25 71.59 81.29 76.41 72.86 62.73 4,4,3,3 aDGlcp 100.10 70.93 75.53 70.91 73.89 60.99 4,4,3 aDManp 102.12 69.54 77.94 70.84 73.14 60.89 4,4 aDManp 101.25 71.59 81.29 76.41 72.86 62.73 4,4,4,4,3,3,4,4,3,3,3 aDGlcp 100.10 70.93 75.53 70.91 73.89 60.99 4,4,4,4,3,3,4,4,3,3 bDGlcp 4,4,4,4,3,3,4,4,3 bDGlcp 101.64 71.62 82.20 69.58 75.46 62.19 4,4,4,4,3,3,4,4 bDGlcp 4,4,4,4,3,3 bDXylp 102.37 74.43 74.48 76.80 63.57 4,4,4,4 aDGalp 101.79 ? 81.52 69.27 75.26 61.30 4,3,3 aDGlcp 100.10 70.93 75.53 70.91 73.89 60.99 4,3 aDManp 102.12 69.54 77.94 70.84 73.14 60.89 4 aDManp 101.25 71.59 81.29 76.41 72.86 62.73 SUG 1H NMR data: Linkage Residue H1 H2 H3 H4 H5 H6 4,4,4,4,3,3,4 aDGlcp 4.94 3.37 3.48 3.56 3.51 3.63 4,4,4,4,3 aDGalp 4.99 3.84 4.02 3.96 3.52 3.73 4,4,4,3,2 aDGlcp 4.89 3.66 3.72 3.43 3.82 3.74 4,4,4,3 aDManp 5.29 3.75 3.84 3.69 3.96 3.69 4,4,4 aDManp 5.19 3.62 3.92 3.82 3.76 3.74 4,4,3,3 aDGlcp 4.89 3.66 3.72 3.43 3.82 3.74 4,4,3 aDManp 5.03 4.22 3.95 3.74 3.71 3.75 4,4 aDManp 5.19 3.62 3.92 3.82 3.76 3.74 4,4,4,4,3,3,4,4,3,3,3 aDGlcp 4.89 3.66 3.72 3.43 3.82 3.74 4,4,4,4,3,3,4,4,3,3 bDGlcp 4,4,4,4,3,3,4,4,3 bDGlcp 4.57 3.61 3.71 4.02 3.65 3.80 4,4,4,4,3,3,4,4 bDGlcp 4,4,4,4,3,3 bDXylp 4.43 3.15 3.40 3.58 3.20 4,4,4,4 aDGalp 4.99 3.84 4.02 3.96 3.52 3.73 4,3,3 aDGlcp 4.89 3.66 3.72 3.43 3.82 3.74 4,3 aDManp 5.03 4.22 3.95 3.74 3.71 3.75 4 aDManp 5.19 3.62 3.92 3.82 3.76 3.74 SUG 1H/13C HSQC data: Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 4,4,4,4,3,3,4 aDGlcp 100.17/4.94 72.45/3.37 74.13/3.48 76.84/3.56 73.20/3.51 61.18/3.63 4,4,4,4,3 aDGalp 101.79/4.99 ?/3.84 81.52/4.02 69.27/3.96 75.26/3.52 61.30/3.73 4,4,4,3,2 aDGlcp 100.10/4.89 70.93/3.66 75.53/3.72 70.91/3.43 73.89/3.82 60.99/3.74 4,4,4,3 aDManp 99.30/5.29 78.65/3.75 71.72/3.84 65.49/3.69 69.68/3.96 60.46/3.69 4,4,4 aDManp 101.25/5.19 71.59/3.62 81.29/3.92 76.41/3.82 72.86/3.76 62.73/3.74 4,4,3,3 aDGlcp 100.10/4.89 70.93/3.66 75.53/3.72 70.91/3.43 73.89/3.82 60.99/3.74 4,4,3 aDManp 102.12/5.03 69.54/4.22 77.94/3.95 70.84/3.74 73.14/3.71 60.89/3.75 4,4 aDManp 101.25/5.19 71.59/3.62 81.29/3.92 76.41/3.82 72.86/3.76 62.73/3.74 4,4,4,4,3,3,4,4,3,3,3 aDGlcp 100.10/4.89 70.93/3.66 75.53/3.72 70.91/3.43 73.89/3.82 60.99/3.74 4,4,4,4,3,3,4,4,3,3 bDGlcp 4,4,4,4,3,3,4,4,3 bDGlcp 101.64/4.57 71.62/3.61 82.20/3.71 69.58/4.02 75.46/3.65 62.19/3.80 4,4,4,4,3,3,4,4 bDGlcp 4,4,4,4,3,3 bDXylp 102.37/4.43 74.43/3.15 74.48/3.40 76.80/3.58 63.57/3.20 4,4,4,4 aDGalp 101.79/4.99 ?/3.84 81.52/4.02 69.27/3.96 75.26/3.52 61.30/3.73 4,3,3 aDGlcp 100.10/4.89 70.93/3.66 75.53/3.72 70.91/3.43 73.89/3.82 60.99/3.74 4,3 aDManp 102.12/5.03 69.54/4.22 77.94/3.95 70.84/3.74 73.14/3.71 60.89/3.75 4 aDManp 101.25/5.19 71.59/3.62 81.29/3.92 76.41/3.82 72.86/3.76 62.73/3.74 SUG
1H NMR data:
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13C NMR data:
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The spectrum also has 2 signals at unknown positions (not plotted). |
SMILES errors: aDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-4)/aDGlcp(1-4)/n=8/bDXylp(1-3)/aDGalp(1-3)/n=3/aDGalp(1-4)/[aDGlcp(1-2)aDManp(1-3)]aDManp(1-4)/n=2//[aDGlcp(1-3)aDManp(1-3)]aDManp(1-4)/n=4/[aDGlcp(1-3)aDManp(1-3)]aDManp(1-4)SUG: SMILES error: could not calculate brutto descriptors of a molecule from SMILES [1*]O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O[C@H]6O[C@H](CO)[C@@H](O[C@H]7O[C@H](CO)[C@@H](O[C@H]8O[C@H](CO)[C@H](O)[C@H](O[C@H]9O[C@H](CO)[C@H](O)[C@H](O[C@H]%10O[C@H](CO)[C@H](O)[C@H](O[C@H]%11O[C@H](CO)[C@H](O)[C@H](O[C@@H]%12OC[C@@H](O[C@H]%13O[C@H](CO)[C@@H](O[C@H]%14O[C@H](CO)[C@@H](O[C@H]%15O[C@H](CO)[C@@H](O[C@H]%16O[C@H](CO)[C@@H](O[C@H]%17O[C@H](CO)[C@@H](O[C@@H]%18O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%19O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%20O[C@H](CO)[C@@H](O)[C@H](O[C@H]%21O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%21O)[C@H]%20O)[C@H]%19O)[C@H]%18O)[C@H](O)[C@H]%17O)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@H](O)[C@H]%14O)[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H]%11O)[C@H]%10O)[C@H]9O)[C@H]8O)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H]7O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@@H]5O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H]2O)[C@@H]1O Invalid SMILES [1*]O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O[C@H]6O[C@H](CO)[C@@H](O[C@H]7O[C@H](CO)[C@@H](O[C@H]8O[C@H](CO)[C@H](O)[C@H](O[C@H]9O[C@H](CO)[C@H](O)[C@H](O[C@H]%10O[C@H](CO)[C@H](O)[C@H](O[C@H]%11O[C@H](CO)[C@H](O)[C@H](O[C@@H]%12OC[C@@H](O[C@H]%13O[C@H](CO)[C@@H](O[C@H]%14O[C@H](CO)[C@@H](O[C@H]%15O[C@H](CO)[C@@H](O[C@H]%16O[C@H](CO)[C@@H](O[C@H]%17O[C@H](CO)[C@@H](O[C@@H]%18O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%19O[C@H](CO)[C@@H](O)[C@H](O[C@@H] O[C@H](CO)[C@@H](O)[C@H](O[C@H]%21O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%21O)[C@H] O)[C@H]%19O)[C@H]%18O)[C@H](O)[C@H]%17O)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@H](O)[C@H]%14O)[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H]%11O)[C@H]%10O)[C@H]9O)[C@H]8O)[C@H](O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H]7O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H]6O)[C@@H]5O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@@H]4O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H]2O)[C@@H]1O
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2. (CSDB ID: 41096) | report error |
| a-D-Galp-(1-6)-+ | a-D-Galp-(1-2)-+ | | | a-D-Galp-(1-6)-a-D-Manp-(1-3)-+ | a-D-Galp-(1-6)-a-D-Manp-(1-4)-+ | | | | | -6)-a-D-Manp-(1-6)-a-D-Glcp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Glcp-(1-6)-a-D-Manp-(1-2)-a-D-Manp-(1- | a-D-Galp-(1-2)-+ | Show graphically |
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Show legend Show as text |
Ophiocordyceps gracilis
(NCBI TaxID 2651840,
species name lookup)
njnu.edu.cn>; H. Huang <huanghnjnu.edu.cn>Ophiocordyceps gracilis (O. gracilis) is a parasitic fungus used in traditional Chinese medicine and functional foods. In this study, a neutral heteropolysaccharide (GSP-1a) was isolated from spores of O. gracilis, and its structure and antioxidant capacities were investigated. GSP-1a was found to have a molecular weight of 72.8 kDa and primarily consisted of mannose (42.28%), galactose (35.7%), and glucose (22.02%). The backbone of GSP-1a was composed of various sugar residues, including →6)-α-D-Manp-(1→, →2,6)-α-D-Manp-(1→, →2,4,6)-α-D-Manp-(1→, →6)-α-D-Glcp-(1→, and →3,6)-α-D-Glcp-(1→, with some branches consisting of →6)-α-D-Manp-(1→ and α-D-Gal-(1→. In vitro, antioxidant activity assays demonstrated that GSP-1a exhibited scavenging effects on hydroxyl radical (•OH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+), and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•). Moreover, GSP-1a was found to alleviate H2O2-induced oxidative stress in HepG2 cells by reducing the levels of reactive oxygen species (ROS) and malondialdehyde (MDA), while enhancing the activities of superoxide dismutase (SOD). Furthermore, GSP-1a upregulated the mRNA expression of antioxidant enzymes such as Ho-1, Gclm, and Nqo1, and regulated the NRF2/KEAP1 and FNIP1/FEM1B pathways. The findings elucidated the structural types of GSP-1a and provided a reliable theoretical basis for its usage as a natural antioxidant in functional foods or medicine.
polysaccharide, spores, Antioxidant activity, oxidative stress, Ophiocordyceps gracilis
Structure type: structural motif or average structure ; 7280013C NMR data: Linkage Residue C1 C2 C3 C4 C5 C6 2,6,6,6,6,6 aDManp 2,6,6,6,6,3,6 aDGalp 97.29 71.24 72.09 72.68 74.37 60.39 2,6,6,6,6,3 aDManp 2,6,6,6,6 aDGlcp 97.24 71.03 86.89 ? 75.31 69.59 2,6,6,6,2 aDGalp 97.29 71.24 72.09 72.68 74.37 60.39 2,6,6,6 aDManp 2,6,6 aDManp 2,6 aDGlcp 99.56 73.06 72.81 ? 75.31 69.46 2,2 aDGalp 97.29 71.24 72.09 72.68 74.37 60.39 2,4,6 aDGalp 97.29 71.24 72.09 72.68 74.37 60.39 2,4 aDManp 2 aDManp 6 aDGalp 97.29 71.24 72.09 72.68 74.37 60.39 aDManp 1H NMR data: Linkage Residue H1 H2 H3 H4 H5 H6 2,6,6,6,6,6 aDManp 5.08 3.95 4.05 3.75 3.78 3.77 2,6,6,6,6,3,6 aDGalp 4.92 3.50 3.74 3.66 4.01 3.71-3.80 2,6,6,6,6,3 aDManp 5.08 3.95 4.05 3.75 3.78 3.77 2,6,6,6,6 aDGlcp 4.98 3.51 4.16 3.94 4.17 3.56-3.91 2,6,6,6,2 aDGalp 4.92 3.50 3.74 3.66 4.01 3.71-3.80 2,6,6,6 aDManp 5.27 4.19 4.11 3.79 3.78 3.75-4.28 2,6,6 aDManp 5.08 3.95 4.05 3.75 3.78 3.77 2,6 aDGlcp 4.84 3.50 3.71 3.94 4.17 3.39-3.83 2,2 aDGalp 4.92 3.50 3.74 3.66 4.01 3.71-3.80 2,4,6 aDGalp 4.92 3.50 3.74 3.66 4.01 3.71-3.80 2,4 aDManp 2 aDManp 5.27 4.19 4.05 3.96 ? 3.76-4.28 6 aDGalp 4.92 3.50 3.74 3.66 4.01 3.71-3.80 aDManp 5.27 4.19 4.11 3.79 3.78 3.75-4.28 1H/13C HSQC data: Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 2,6,6,6,6,6 aDManp NMR TSV error 2: unequal length of 13C and 1H datasets 2,6,6,6,6,3,6 aDGalp 97.29/4.92 71.24/3.50 72.09/3.74 72.68/3.66 74.37/4.01 60.39/3.71-3.80 2,6,6,6,6,3 aDManp NMR TSV error 2: unequal length of 13C and 1H datasets 2,6,6,6,6 aDGlcp 97.24/4.98 71.03/3.51 86.89/4.16 ?/3.94 75.31/4.17 69.59/3.56-3.91 2,6,6,6,2 aDGalp 97.29/4.92 71.24/3.50 72.09/3.74 72.68/3.66 74.37/4.01 60.39/3.71-3.80 2,6,6,6 aDManp NMR TSV error 2: unequal length of 13C and 1H datasets 2,6,6 aDManp NMR TSV error 2: unequal length of 13C and 1H datasets 2,6 aDGlcp 99.56/4.84 73.06/3.50 72.81/3.71 ?/3.94 75.31/4.17 69.46/3.39-3.83 2,2 aDGalp 97.29/4.92 71.24/3.50 72.09/3.74 72.68/3.66 74.37/4.01 60.39/3.71-3.80 2,4,6 aDGalp 97.29/4.92 71.24/3.50 72.09/3.74 72.68/3.66 74.37/4.01 60.39/3.71-3.80 2,4 aDManp 2 aDManp NMR TSV error 2: unequal length of 13C and 1H datasets 6 aDGalp 97.29/4.92 71.24/3.50 72.09/3.74 72.68/3.66 74.37/4.01 60.39/3.71-3.80 aDManp NMR TSV error 2: unequal length of 13C and 1H datasets
1H NMR data:
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13C NMR data:
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The spectrum also has 2 signals at unknown positions (not plotted). |
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Execution: 5 sec