CSDB: Search results

Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting bodies

The structure was elucidated in this paper
Publication DOI: 10.1016/j.foodhyd.2020.106568
Journal NLM ID: 8701770
Publisher: New York, NY: Elsevier
Correspondence: S.P. Nie <spnie

ncu.edu.cn>; <nie68

sina.com>
Institutions: State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi Province, 330047, China

Polysaccharide from Agrocybe cylindracea has been demonstrated to exhibit excellently biological functions, but insufficient understanding of its physicochemical property and fine structure limits its wide application and development. In the present study, two water-soluble heteroglycans, a high molecular weight (SACP-40) and a low molecular weight (SACP-80), were isolated from the fruit bodies of A. cylindracea by hot water extraction and gradient ethanol precipitation. There was little difference in their physicochemical properties, including major chemicals and monosaccharide compositions. Rheological measurements revealed SACP-80 was a typical pseudoplastic fluid with shear thinning behavior at the concentration of 3.0%, whereas exhibited a near-Newtonian fluid at lower concentration. SEM observation suggested SACP-80 showed micro-spherical shapes loosely attached to fibrous filament in its surface. Besides, methylation and 1D/2D NMR analysis indicated that SACP-80 was a branched heteroglycan with a backbone composed of β-(1→6)-glucopyranosyl, α-(1→6)-galactopyranosyl and α-(1→2,6)-glucopyranosyl residue at a ratio of 3:1:1. This work provides a theoretical basis for gaining insight into the relationship between the fine structure and viscosity of polysaccharide from A. cylindracea, which could further guide its functional studies and advance the potential application in food and biomedicine industries.

Agrocybe cylindracea, structural characteristic, heteroglycans, viscosity and morphology

Structure type: structural motif or average structure ; 156000
Location inside paper: table 3, Fig. 6, SACP-80
Trivial name: polysaccharide SACP-80, fucoglucogalactan (ACP), polysaccharide SACP-80
Contained glycoepitopes: IEDB_115015,IEDB_134624,IEDB_135614,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_152214,IEDB_153553,IEDB_167069,IEDB_174333,IEDB_190606,IEDB_241101,IEDB_983931,SB_154,SB_163,SB_192,SB_7,SB_86

Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, FTIR, composition analysis, HPLC, HPSEC-MALLS, HPAEC-PAD, rheological measurements, statistical analysis, HPGPC, SEM, hot water extraction, gradient ethanol precipitation
Comments, role: Proposed structure of SACP-80 from A. cylindrace.

NCBI Taxonomy refs (TaxIDs): 1593264
Show glycosyltransferases

NMR conditions: in D2O at 298 K [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
6,6,6,6	a?Galp	97.87	70.06	69.69	69.11	68.75	67.22
6,6,6	a?Galp	97.95	78.18	69.11	68.86	67.30	66.97
6,6	b?Glcp	102.86	73.03	75.43	?	?	?
6	b?Glcp
	b?Glcp	102.86	73.03	75.43	69.44	75.01	68.68
6,6,6,2	a?Fucp	101.46	70.47	70.06	?	?	15.63
6,6,6,2	b?Glcp	102.32	72.78	74.68	68.58	75.87	60.54


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
6,6,6,6	a?Galp	4.87	3.70	3.91	4.08	4.05	3.85
6,6,6	a?Galp	4.95	3.70	3.96	4.00	4.11	3.88
6,6	b?Glcp	4.410	3.14	3.35	?	?	?
6	b?Glcp
	b?Glcp	4.41	3.26	3.40	3.34	3.52	3.74-4.02
6,6,6,2	a?Fucp	4.99	3.388	3.92	?	?	1.11
6,6,6,2	b?Glcp	4.65	3.33	3.48	3.45	3.38	3.67-3.81


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
6,6,6,6	a?Galp	97.87/4.87	70.06/3.70	69.69/3.91	69.11/4.08	68.75/4.05	67.22/3.85
6,6,6	a?Galp	97.95/4.95	78.18/3.70	69.11/3.96	68.86/4.00	67.30/4.11	66.97/3.88
6,6	b?Glcp	102.86/4.410	73.03/3.14	75.43/3.35	?/?	?/?	?/?
6	b?Glcp
	b?Glcp	102.86/4.41	73.03/3.26	75.43/3.40	69.44/3.34	75.01/3.52	68.68/3.74-4.02
6,6,6,2	a?Fucp	101.46/4.99	70.47/3.388	70.06/3.92	?/?	?/?	15.63/1.11
6,6,6,2	b?Glcp	102.32/4.65	72.78/3.33	74.68/3.48	68.58/3.45	75.87/3.38	60.54/3.67-3.81

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
6,6,6,6	a?Galp	4.87	3.70	3.91	4.08	4.05	3.85
6,6,6	a?Galp	4.95	3.70	3.96	4.00	4.11	3.88
6,6	b?Glcp	4.410	3.14	3.35	?	?	?
6	b?Glcp
	b?Glcp	4.41	3.26	3.40	3.34	3.52	3.74 4.02
6,6,6,2	a?Fucp	4.99	3.388	3.92	?	?	1.11
6,6,6,2	b?Glcp	4.65	3.33	3.48	3.45	3.38	3.67 3.81

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
6,6,6,6	a?Galp	97.87	70.06	69.69	69.11	68.75	67.22
6,6,6	a?Galp	97.95	78.18	69.11	68.86	67.30	66.97
6,6	b?Glcp	102.86	73.03	75.43	?	?	?
6	b?Glcp
	b?Glcp	102.86	73.03	75.43	69.44	75.01	68.68
6,6,6,2	a?Fucp	101.46	70.47	70.06	?	?	15.63
6,6,6,2	b?Glcp	102.32	72.78	74.68	68.58	75.87	60.54

The spectrum also has 5 signals at unknown positions (not plotted).

There are 64 chemically distinct structures. Please, select:

*OC[C@H]1O[C@H](OC[C@H]2O[C@H](OC[C@H]3O[C@@H](OC[C@H]4O[C@@H](OC[C@H]5O[C@@H](*)[C@H](O)[C@@H](O)[C@@H]5O)[C@H](O)[C@@H](O)[C@@H]4O)[C@H](O)[C@@H](O)[C@@H]3O)[C@H](O[C@H]3O[C@H](C)[C@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize