Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting bodies
NCBI PubMed ID: 37364942Publication DOI: 10.1016/j.carbpol.2023.121108Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: K. Wang <wkpzcq
163.com>
Institutions: Hubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, 430030 Wuhan, China, Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China, Hubei province clinical research center for precision medicine for critical illness, 430030 Wuhan, China, Department of Pharmacy, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, 430019 Wuhan, China
It had been shown that lentinan (LNT) was mainly distributed in the liver after intravenous administration. The study aimed to investigate the integrated metabolic processes and mechanisms of LNT in the liver, as these have not been thoroughly explored. In current work, 5-([4,6-dichlorotriazin-2-yl] amino) fluorescein and cyanine 7 were used to label LNT for tracking its metabolic behavior and mechanisms. Near-infrared imaging demonstrated that LNT was captured mainly by the liver. Kupffer cell (KC) depletion reduced LNT liver localization and degradation in BALB/c mice. Moreover, experiments with Dectin-1 siRNA and Dectin-1/Syk signaling pathway inhibitors showed that LNT was mainly taken up by KCs via the Dectin-1/Syk pathway and promoted lysosomal maturation in KCs via this same pathway, which in turn promoted LNT degradation. These empirical findings offer novel insights into the metabolism of LNT in vivo and in vitro, which will facilitate the further application of LNT and other beta-glucans.
lentinan, fluorescent labeling, Dectin-1, Kupffer cells, metabolic degradation
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 2A, LNT (lentinan)
Trivial name: lentinan
Compound class: glucan, polysaccharide
Methods: Western blotting, HPLC, extraction, statistical analysis, flow cytometry analysis, fluorescence labeling, HPGPC, immunohistochemistry, metabolic assay, cellular uptake and degradation of FLNT
Biological activity: LNT was first phagocytosed via the Dectin-1/Syk signaling pathway in the hepatic KCs and then promoted the maturation of phagosomes in KCs through the Dectin-1/Syk signaling pathway, which in turn fostered the degradation of LNT
NCBI Taxonomy refs (TaxIDs): 5353
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error
(later renamed to: Lentinula edodes)