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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Organ / tissue: blastospores, hypha

The structure was elucidated in this paper
NCBI PubMed ID: 12829394
Publication DOI: 10.1016/S0008-6215(03)00169-1
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: dwlowman

eastman.com
Institutions: Global Polymers and Research Analytical Services, Eastman Chemical Company, Kingsport, TN 37662-5150, USA, Department of Surgery, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA, Department of Microbiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA - ALL AFFILIATIONS SHOULD BE LISTED, Department of Microbiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA

Glucans are (1→3)-β-linked linear and branched polymers containing anhydroglucose repeat units. They comprise a major portion of the cell wall of saprophytic and pathogenic fungi. Glucans activate a wide range of innate immune responses. They are also released from the fungal cell wall as exopolymers into the blood of patients with fungal infections. Extensive studies have been done on glucans isolated from saprophytic fungi, such as Saccharomyces cerevisiae; however, much less is known about the glucans produced by the polymorphic fungal pathogen Candida albicans. We have undertaken an extensive structural characterization and comparison of glucans isolated from C. albicans blastospores and hyphae using high-resolution, solution-state proton nuclear magnetic resonance spectroscopy (NMR). In addition, we developed a simple and straightforward method for the production of Candida hyphae that resulted in gram quantities of hyphal mass. Also, we compared and contrasted the Candida glucans isolated by two different protocols with those isolated from S. cerevisiae. Isolation protocols provide high purity glucans with source-based structural differences. Structural details provided by this NMR analysis included the degree of polymerization, molecular weight, degree and type of branching, and structural composition. We observed that Candida glucans, derived from blastospores or hyphae, are different compared to those isolated from S. cerevisiae with regard to side-chain branching along the backbone and at the reducing terminus. These structural details are an important prerequisite for biomedical studies on the interaction of isolated fungal cell wall glucans with the innate immune system.

NMR, pathogen, Candida albicans, Blastospore, Hyphae

Structure type: structural motif or average structure
Location inside paper: p.1492, fig.1, structure 2
Trivial name: β-1,3-D-glucan
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_241101,IEDB_983931,SB_192

Methods: 1H NMR, HPSEC-MALLS, extraction
Comments, role: water-insoluble (1-3)-b-D-glucan with shortest backbone and side-chain attached to reducing terminus exclusively at C6-position; 1,3-b-D-glucan chain may be longer.

Related record ID(s): 41209, 41210
NCBI Taxonomy refs (TaxIDs): 5476
Reference(s) to other database(s): GTC:G24736HD
Show glycosyltransferases

There is only one chemically distinct structure:

OC[C@H]1O[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@@H]3[C@@H](O)[C@H](O[C@@H]4[C@@H](O)C(O)O[C@H](CO[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H]4O)O[C@H](CO)[C@H]3O)O[C@H](CO)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize